Specific hemagglutinins in x-irradiated, bone-marrow treated mice following differential immunization of host and donor.
Specific hemagglutinins in x-irradiated, bone-marrow treated mice following differential immunization of host and donor.
复制标题
宿主和供体差异免疫后，X射线照射、骨髓处理的小鼠体内的特异性血凝素。
作者：
R. M. Garver;G. Santos;L. J. Cole
对致敏小鼠组进行照射（870 r），并注射来自先前用其他抗原致敏的供体的小鼠或大鼠骨髓。在其他实验中，受到致命辐射的小鼠仅注射同源骨髓，宿主或骨髓供体都被致敏；或者谁都不敏感。每个实验组在辐射和骨髓保护后的不同时间接受“加强”抗原注射。照射后定期测定所有组的抗羊红细胞和抗人红细胞凝集素滴度。
结果如下： a) 如果在照射前致敏，所有骨髓处理的受照射小鼠都继续产生抗体； b) 经过同源骨髓治疗、预敏化的小鼠对“加强剂”产生二次反应；异源骨髓治疗的小鼠则没有； c) 同源骨髓治疗的小鼠能够产生初级反应，但异源骨髓治疗的小鼠则不能。
结果可以解释为以下证据： a) 主要反应是放射敏感的；抗体的产生相对不敏感； b) 二次免疫反应具有相对抗辐射性； c) 使用同源骨髓治疗可使“同源嵌合体”恢复基本正常的免疫反应。在异源嵌合体中，免疫反应始终处于非常低的水平。
Groups of sensitized mice were irradiated (870 r) and injected with mouse or rat bone marrow from donors previously sensitized with the other antigen. In other experiments, lethally irradiated mice were injected with isologous bone marrow only, and either the host or the bone marrow donor was sensitized; or neither one was sensitized. Groups from each experiment were given “booster” injections of antigen at various times subsequent to irradiation and bone marrow protection. Anti-sheep-erythrocyte and antihuman-erythrocyte agglutinin titers were determined on all groups periodically after irradiation.
The results were as follows: a) All marrow-treated irradiated mice continued to produce antibodies if sensitized prior to irradiation; b) The isologous bone marrow treated, presensitized mice reacted to a “booster” with a secondary response; the heterologous bone marrow treated mice did not; c) The isologous bone marrow treated mice were capable of a primary response but the heterologous bone marrow treated mice were not.
The results may be interpreted as evidence that: a) The primary response is radiosensitive; antibody production is relatively insensitive; b) The secondary immune response is relatively radioresistant; c) treatment with isologous bone marrow leads to recovery of essentially normal immune response in the “isologous chimera.” In the heterologous chimera, the immune response, is at all times, at a very low level.