Development of Surface Plasmon Resonance Imaging Apparatus for High-Throughput Study of Protein-Surface Interactions
Development of Surface Plasmon Resonance Imaging Apparatus for High-Throughput Study of Protein-Surface Interactions
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DOI：
10.1380/ejssnt.2006.201
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发表时间：
2006-01-01
影响因子：
0.7
通讯作者：
Iwata, Hiroo
中科院分区：
文献类型：
--
作者：
Arima, Yusuke;Ishii, Rika;Iwata, Hiroo
我们报告了使用呈现具有不同表面特性的斑点的阵列和表面等离子共振（SPR）成像设备对蛋白质-表面相互作用的并行分析。该阵列是通过对带有不同官能团（包括甲基、羟基、羧酸、胺和聚（乙二醇）束缚胺）的自组装单层（SAM）进行光图案化来制造的。 SPR 成像设备是内部开发的。通过 SPR 成像监测 SAM 上牛血清白蛋白 (BSA) 的吸附行为。使用SPR成像装置获得的吸附量与通过二辛可宁酸(BCA)测定获得的吸附量具有良好的相关性。该阵列与SPR成像的结合适用于蛋白质与各种特性表面相互作用的高通量、实时、无标记和高灵敏度研究。
We report a parallel analysis of protein-surface interactions using an array presenting spots with different surface properties and a surface plasmon resonance (SPR) imaging apparatus. The array was fabricated by the photopatterning of self-assembled monolayers (SAMs) carrying different functional groups including methyl, hydroxyl, carboxylic acid, amine, and poly(ethylene glycol)-tethered amine. The SPR imaging apparatus was developed in-house. The adsorption behavior of bovine serum albumin (BSA) on SAMs was monitored by SPR imaging. The adsorption amounts obtained using the SPR imaging apparatus were well correlated with those obtained by the bicinchoninic acid (BCA) assay. The combination of the array and SPR imaging is suitable for high-throughput, real-time, label-free, and highly-sensitive studies of the interactions of proteins with surfaces with various properties.