Genetic compensation by epob in pronephros development in epoa mutant zebrafish.
Genetic compensation by epob in pronephros development in epoa mutant zebrafish.
复制标题
epob 在 epoa 突变斑马鱼前肾发育中的遗传补偿。
DOI：
10.1080/15384101.2019.1656019
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发表时间：
2019
期刊：
影响因子：
4.3
通讯作者：
Kroll Jens
中科院分区：
文献类型：
--
作者：
She Jianqing;Wu Yue;Lou Bowen;Lodd Elisabeth;Klems Alina;Schmoehl Felix;Yuan Zuyi;Le Noble Ferdin;Kroll Jens
斑马鱼促红细胞生成素a（epoa）是一种特性良好的红细胞形成调节剂。最近的吗啉代介导的敲除数据也确定了对斑马鱼的生理性原肾发育至关重要的抑制剂，其通过阻断发育中的肾脏中的细胞凋亡来驱动。然而，斑马鱼突变体forepoa至今尚未被描述。为了比较瞬时敲除与永久敲除前肾对斑马鱼前肾发育的影响，我们利用CRISPR/Cas9技术制备了前肾敲除斑马鱼突变体，并对前肾发育进行了结构和功能研究。与epoamorphants相反，epoa−/-斑马鱼突变体显示出正常的原肾结构;然而，在斑马鱼中发现了一个以前未表征的基因，命名为depob，并上调了inepoa −/-突变体。同样，epoaandepobmorphants调节相似和差异的基因签名相关的斑马鱼肾脏发育。总之，胚胎发育过程中的稳定性缺失可以通过基因敲除和基因敲除的斑马鱼胚胎中表型差异来补偿。
Zebrafish erythropoietin a (epoa) is a well characterized regulator of red blood cell formation. Recent morpholino mediated knockdown data have also identifiedepoabeing essential for physiological pronephros development in zebrafish, which is driven by blocking apoptosis in developing kidneys. Yet, zebrafish mutants forepoahave not been described so far. In order to compare a transient knockdown vs. permanent knockout forepoain zebrafish on pronephros development, we used CRISPR/Cas9 technology to generateepoaknockout zebrafish mutants and we performed structural and functional studies on pronephros development. In contrast toepoamorphants,epoa−/-zebrafish mutants showed normal pronephros structure; however, a previously uncharacterized gene in zebrafish, namedepob, was identified and upregulated inepoa−/-mutants.epobknockdown altered pronephros development, which was further aggravated inepoa−/-mutants. Likewise,epoaandepobmorphants regulated similar and differential gene signatures related to kidney development in zebrafish. In conclusion, stable loss ofepoaduring embryonic development can be compensated byepobleading to phenotypical discrepancies inepoaknockdown and knockout zebrafish embryos.