Projections from basal forebrain to prefrontal cortex comprise cholinergic, GABAergic and glutamatergic inputs to pyramidal cells or interneurons
Projections from basal forebrain to prefrontal cortex comprise cholinergic, GABAergic and glutamatergic inputs to pyramidal cells or interneurons
复制标题
DOI：
10.1111/j.1460-9568.2008.06029.x
复制
发表时间：
2008-02-01
影响因子：
3.4
通讯作者：
Jones, Barbara E.
中科院分区：
文献类型：
--
作者：
Henny, Pablo;Jones, Barbara E.
本研究进行了表征的前和突触后成分的基底前脑（BF）的投射到前额叶皮层的大鼠，并确定它是否包括amatergic除了建立γ-氨基丁酸（GABA）能和胆碱能元素。用生物素葡聚糖胺（BDA）标记BF纤维顺行转运，并对谷氨酸（VGluT）、GABA（VGAT）或乙酰胆碱（VAChT）的囊泡转运蛋白（VTP）进行双重染色。通过荧光显微镜观察并通过体视学估计，发现BDA标记的静脉曲张的比例被VGluT 2（而不是VGluT 1或3）、VGAT或VAChT染色（分别代表边缘下皮质内的15%、52%和19%）。每种类型都存在于所有的，虽然通常最密集的深层，皮质层。对材料进行突触后蛋白的三重染色，以检查BDA+VTP+静脉曲张是否可能分别形成兴奋性或抑制性突触，由突触后密度-95 kDA（PSD-95）或桥蛋白（Geph）标记。通过共聚焦显微镜观察，发现大多数BDA+/VGluT 2+静脉曲张与PSD-95+元件并置，并且大多数BDA+/VGAT+静脉曲张与Geph+元件并置。对其他系列的细胞标记蛋白进行三重染色，以评估静脉曲张是否接触中间神经元或锥体细胞。通过共聚焦显微镜观察，发现BDA标记的VGluT 2+、VGAT+和VAChT+ BF终末均与钙结合蛋白+中间神经元接触，而VGAT+ BF终末也与小白蛋白+中间神经元和非磷酸化神经丝+锥体细胞接触。通过不同的多巴胺能、GABA能和胆碱能投射，BF可以以不同的方式影响皮质活动。
The present study was undertaken to characterize the pre- and postsynaptic constituents of the basal forebrain (BF) projection to the prefrontal cortex in the rat, and determine whether it includes glutamatergic in addition to established gamma-aminobutyric acid (GABA)ergic and cholinergic elements. BF fibres were labelled by anterograde transport using biotin dextran amine (BDA) and dual-stained for the vesicular transporter proteins (VTPs) for glutamate (VGluT), GABA (VGAT) or acetylcholine (VAChT). Viewed by fluorescence microscopy and estimated by stereology, proportions of BDA-labelled varicosities were found to be stained for VGluT2 (and not VGluT1 or 3), VGAT or VAChT (representing, respectively, similar to 15%, similar to 52% and similar to 19% within the infralimbic cortex). Each type was present in all, though commonly most densely in deep, cortical layers. Material was triple-stained for postsynaptic proteins to examine whether BDA+VTP+ varicosities might form excitatory or inhibitory synapses, respectively, labelled by postsynaptic density-95 kDA (PSD-95) or gephyrin (Geph). Viewed by confocal microscopy, a majority of BDA+/VGluT2+ varicosities were found to be apposed to PSD-95+ elements, and a majority of BDA+/VGAT+ varicosities to be apposed to Geph+ elements. Other series were triple-stained for cell marker proteins to assess whether the varicosities contacted interneurons or pyramidal cells. Viewed by confocal microscopy, BDA-labelled VGluT2+, VGAT+ and VAChT+ BF terminals were all found in contact with calbindin+ interneurons, whereas VGAT+ BF terminals were also seen in contact with parvalbumin+ interneurons and non-phosphorylated neurofilament+ pyramidal cells. Through distinct glutamatergic, GABAergic and cholinergic projections, the BF can thus influence cortical activity in a diverse manner.