Quantum-enhanced stimulated Raman scattering microscopy toward breaking the shot noise limit in high-power regime
Quantum-enhanced stimulated Raman scattering microscopy toward breaking the shot noise limit in high-power regime
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DOI：
10.1117/12.2649579
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发表时间：
2023-03
期刊：
影响因子：
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通讯作者：
K. Oguchi;Zicong Xu;Yoshitaka Taguchi;Shunzo Takahashi;Y. Sano;T. Mizuguchi;K. Katoh;Y. Ozeki
中科院分区：
文献类型：
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作者：
K. Oguchi;Zicong Xu;Yoshitaka Taguchi;Shunzo Takahashi;Y. Sano;T. Mizuguchi;K. Katoh;Y. Ozeki
受激拉曼散射(SRS)显微镜是一种强大的技术，它允许将分子振动图像可视化，用于无标记成像、代谢成像和超复合成像。然而，它的灵敏度主要受激光脉冲的散粒噪声的限制，有时需要较长的捕获时间才能检测到被散粒噪声掩盖的微弱SRS信号。为了克服这一限制，量子增强(QE-)受激拉曼散射显微镜已经被证明，而压缩脉冲的低光功率限制了灵敏度。在这项工作中，我们提出了基于量子增强平衡检测(QE-BD)的QE-SRS显微镜，其中平衡检测SRS显微镜的灵敏度通过注入压缩真空来增强，从而允许使用高功率SRS泵浦脉冲(通常为几十毫瓦)进行QE-SRS成像，而平衡检测导致信噪比下降3dB。实验表明，QE-SRS成像与散粒噪声受限的平衡检测SRS相比，噪声降低了2.6dB。我们还演示了通过斯托克斯脉冲的快速波长调谐来实现高光谱QE-SRS成像。这些结果表明了高功率QE-SRS的潜在可行性，它的灵敏度超过了经典的散粒噪声限制的SRS显微镜。
Stimulated Raman scattering (SRS) microscopy is a powerful technique that allows for the visualization of molecular vibrational images for label-free imaging, metabolic imaging, and supermultiplex imaging. However, its sensitivity is mainly limited by the shot noise of laser pulses, and long acquisition times are sometimes required to detect weak SRS signals hidden by the shot noise. To overcome this limitation, quantum-enhanced (QE-) SRS microscopy has been demonstrated, while the low optical power of squeezed pulses limits the sensitivity. In this work, we present QE-SRS microscopy using quantum-enhanced balanced detection (QE-BD) scheme, where the sensitivity of balanced detection SRS microscope is enhanced by injecting squeezed vacuum, allowing for QE-SRS imaging with high power SRS pump pulses (typically several tens of milliwatt), while balanced detection causes 3 dB drawback in the signal-to-noise ratio. We experimentally demonstrate QE-SRS imaging with 2.6 dB noise reduction compared with shot-noise-limited balanced detection SRS. We also demonstrate hyperspectral QE-SRS imaging by fast wavelength tuning of Stokes pulses. These results show the potential feasibility of high-power QE-SRS whose sensitivity is beyond that of classical shot-noise-limited SRS microscopes.