LncRNA-KAT7 Negatively Regulates miR-10a Through Epigenetic Pathway to Participate in Nonsmall Cell Lung Cancer
LncRNA-KAT7 Negatively Regulates miR-10a Through Epigenetic Pathway to Participate in Nonsmall Cell Lung Cancer
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DOI：
10.1089/cbr.2019.3228
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发表时间：
2020-05-18
影响因子：
3.4
通讯作者：
Mu, Lin
中科院分区：
文献类型：
--
作者：
Gao, Yan;Zhao, Hong;Mu, Lin
目的：LncRNA-KAT 7是近年来发现的一种结直肠癌抑癌基因，其在其他恶性肿瘤中的作用尚不清楚。本研究旨在探讨KAT 7在非小细胞肺癌（nonsmall cell lung cancer，NSCLC）中的作用。NSCLC中KAT 7与miR-10a呈负相关。在NSCLC细胞中，KAT 7的过表达导致miR-10a下调，而KAT 7的沉默导致miR-10a上调。甲基化特异性聚合酶链反应显示KAT 7正调控miR-10a的甲基化。细胞增殖实验显示miR-10a过表达可导致NSCLC细胞增殖率增加。结论：KAT 7通过表观遗传学机制负调控miR-10a参与NSCLC细胞增殖。
Objective: LncRNA-KAT7 is a recently identified tumor suppressor in colorectal cancer, whereas its roles in other malignancies remain unclear. This study aimed to investigate the roles of KAT7 nonsmall cell lung cancer (NSCLC).Results: The results showed that KAT7 was downregulated in NSCLC and predicted poor survival. KAT7 is negatively correlated with miR-10a in NSCLC. In NSCLC cells, overexpression of KAT7 led to downregulated miR-10a, whereas silencing of KAT7 led to upregulated miR-10a. Methylation-specific polymerase chain reaction revealed that KAT7 positively regulated the methylation of miR-10a. Cell proliferation assay showed that overexpression of miR-10a led to increased proliferation rate of NSCLC cells. In addition, overexpression of KAT7 played an opposite role and reduced the effects of the overexpression of miR-10a.Conclusion: In conclusion, KAT7 negatively regulates miR-10a through epigenetic mechanisms to participate in NSCLC cell proliferation.