QTL mapping of powdery mildew resistance in WI 2757 cucumber (Cucumis sativus L.)
QTL mapping of powdery mildew resistance in WI 2757 cucumber (Cucumis sativus L.)
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DOI：
10.1007/s00122-013-2125-6
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发表时间：
2013-08-01
影响因子：
5.4
通讯作者：
Weng, Yiqun
中科院分区：
文献类型：
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作者：
He, Xiaoming;Li, Yuhong;Weng, Yiqun
白粉病（PM）是黄瓜（Cucumis sativus L.）的一种非常重要的病害。抗性品种已在生产中广泛应用，但黄瓜抗PM的遗传机制尚不清楚。对源自两个黄瓜自交系 WI 2757（抗性）和 True Lemon（易感）的 132 个 F-2:3 家系进行了为期 3 年的 PM 抗性 QTL 作图研究。绘制了覆盖 610.4 cM、七个连锁群、240 个 SSR 标记位点的遗传图谱。对PM接种反应的下胚轴、子叶和真叶的分子标记数据和疾病指数进行多重QTL作图分析，确定了WI 2757中4条染色体中含有PM抗性QTL的6个基因组区域。在6个QTL中，1号染色体上的pm1.1和pm1.2赋予叶抗性。次要 QTL pm3.1（第 3 号染色体）和 pm4.1（第 4 号染色体）导致疾病易感性。两个主要QTL pm5.1 和pm5.2 位于5 号染色体上大约40 cM 的区间内，每个QTL 解释21.0-74.5 %的表型变异。本文提供的数据支持 5 号染色体中的两个隐性遗传、连锁的主要 QTL 以及其他染色体中的次要 QTL，它们控制着 WI 2757 中的 PM 抗性。下胚轴抗性的 QTL pm5.2 在宿主抗性中起着最重要的作用。同年的多次观察揭示了评分时间在PM抗性QTL检测中的重要性。这项研究的结果为黄瓜白粉病抗性的表型和遗传机制提供了新的见解。
Powdery mildew (PM) is a very important disease of cucumber (Cucumis sativus L.). Resistant cultivars have been deployed in production for a long time, but the genetic mechanisms of PM resistance in cucumber are not well understood. A 3-year QTL mapping study of PM resistance was conducted with 132 F-2:3 families derived from two cucumber inbred lines WI 2757 (resistant) and True Lemon (susceptible). A genetic map covering 610.4 cM in seven linkage groups was developed with 240 SSR marker loci. Multiple QTL mapping analysis of molecular marker data and disease index of the hypocotyl, cotyledon and true leaf for responses to PM inoculation identified six genomic regions in four chromosomes harboring QTL for PM resistance in WI 2757. Among the six QTL, pm1.1 and pm1.2 in chromosome 1 conferred leaf resistance. Minor QTL pm3.1 (chromosome 3) and pm4.1 (chromosome 4) contributed to disease susceptibility. The two major QTL, pm5.1 and pm5.2 were located in an interval of similar to 40 cM in chromosome 5 with each explaining 21.0-74.5 % phenotypic variations. Data presented herein support two recessively inherited, linked major QTL in chromosome 5 plus minor QTL in other chromosomes that control the PM resistance in WI 2757. The QTL pm5.2 for hypocotyl resistance plays the most important role in host resistance. Multiple observations in the same year revealed the importance of scoring time in the detection of PM resistance QTL. Results of this study provided new insights into phenotypic and genetic mechanisms of powdery mildew resistance in cucumber.