Role of Bassoon and Piccolo in Assembly and Molecular Organization of the Active Zone.
Role of Bassoon and Piccolo in Assembly and Molecular Organization of the Active Zone.
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DOI：
10.3389/fnsyn.2015.00019
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发表时间：
2015
影响因子：
3.7
通讯作者：
Garner CC
中科院分区：
文献类型：
--
作者：
Gundelfinger ED;Reissner C;Garner CC
Bason和Piccolo是Cytomatrix的两个非常大的支架蛋白，聚集在神经递质释放的活动区(CAZ)。它们共享沿其整个长度分布的高序列相似性的区域，并且似乎在组织CAZ中共享重叠和不同的功能。在这里，我们综述了我们对蛋白质-蛋白质相互作用的现有知识以及对这两个巨型蛋白质分子功能的理解的最新进展。这些作用包括活动区(AZ)的组装、电压门控钙通道(VGCC)在释放部位附近的定位、突触小泡(SV)的启动以及在Piccolo的情况下，在肌动蛋白细胞骨架的动态组装中的作用。短笛和巴松管对于维持突触前结构和功能，以及对CAZ特化的组装，如突触带，也是重要的。最近的发现表明，它们还参与了突触前突触和神经元核之间依赖活动的调节通讯。总之，这些观察结果表明，巴松管和短笛使用它们的模块化结构来组织对突触前功能的各个方面至关重要的超分子复合体。
Bassoon and Piccolo are two very large scaffolding proteins of the cytomatrix assembled at the active zone (CAZ) where neurotransmitter is released. They share regions of high sequence similarity distributed along their entire length and seem to share both overlapping and distinct functions in organizing the CAZ. Here, we survey our present knowledge on protein-protein interactions and recent progress in understanding of molecular functions of these two giant proteins. These include roles in the assembly of active zones (AZ), the localization of voltage-gated Ca2+ channels (VGCCs) in the vicinity of release sites, synaptic vesicle (SV) priming and in the case of Piccolo, a role in the dynamic assembly of the actin cytoskeleton. Piccolo and Bassoon are also important for the maintenance of presynaptic structure and function, as well as for the assembly of CAZ specializations such as synaptic ribbons. Recent findings suggest that they are also involved in the regulation activity-dependent communication between presynaptic boutons and the neuronal nucleus. Together these observations suggest that Bassoon and Piccolo use their modular structure to organize super-molecular complexes essential for various aspects of presynaptic function.