Prosomes (proteasomes) changes during differentiation are related to the type of inducer
Prosomes (proteasomes) changes during differentiation are related to the type of inducer
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DOI：
10.1023/a:1006856707793
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发表时间：
1997-03-01
影响因子：
2.8
通讯作者：
Chateau, MT
中科院分区：
文献类型：
--
作者：
Bureau, JP;Henry, L;Chateau, MT
26S蛋白酶体的核心20S蛋白是一种高度组织化的多蛋白复合物，在恶性细胞中大量存在。几种细胞系的分化，包括单核细胞U937和淋巴母细胞样细胞CCRF-CEM，在使用磷酸肉豆蔻酸-乙酸酯（PMA）和视黄酸加二羟基维生素D3 （RAS-VD）时，伴随着prosome浓度的普遍降低。U937细胞与PMA或RA+VD孵育3天后，细胞内和质膜上的prosome标记模式不相同。而当PMA作为诱导剂时，U937和CCRF-CEM细胞中也发生了类似的蛋白变化。胞内分布的prosomes也与恶性肿瘤和分化有关。蛋白体存在于癌细胞的细胞核和细胞质中；RA+VD使核内的蛋白体减少，而PMA引起各种蛋白体的改变。这些结果表明，蛋白质在细胞调控和恶性肿瘤的表达中起重要作用。
The core of the 26S proteasome, the 20S prosome, is a highly organized multi-protein complex found in large amount in malignant cells. Differentiation of several cell lines, including the monoblastic U937 and the lymphoblastoid CCRF-CEM, is accompanied by a general decrease in the prosome concentration when phorbol-myrirtic-acetate (PMA) and retinoic acid plus dihydroxyvitamine D3 (RAS-VD) are used. Incubation of U937 cells for three days with PMA or RA+VD causes differentiation, but the resulting patterns of prosome labeling in the cell and on the plasma membrane are not the same. In contrast, the same kind of prosome changes occur in U937 and CCRF-CEM cells when PMA is used as inducer. The intracellular distribution of prosomes is also linked to malignancy and differentiation. Prosomes are found in the nucleus and the cytoplasm of cancer cells; and treatment with RA+VD decreases the prosomes in the nucleus whereas PMA causes various prosome proteins changes. These results indicate that prosomes are important in cell regulation and in the expression of malignancy.