Tail regenerative capacity and iNOS immunolocalization in Xenopus laevis tadpoles
Tail regenerative capacity and iNOS immunolocalization in Xenopus laevis tadpoles
复制标题
DOI：
10.1007/s00441-011-1136-3
复制
发表时间：
2011-05-01
影响因子：
3.6
通讯作者：
Bertolotti, Evelina
中科院分区：
文献类型：
--
作者：
Franchini, Antonella;Bertolotti, Evelina
本文研究了不同年龄非洲爪蟾（Xenopus laevis）幼仔再生尾的形态学和诱导型一氧化氮合酶（inducible isoform of nitric oxide synthase，iNOS）的免疫分布。通过比较阶段-50和阶段-55/56蝌蚪，各种形态学方面和免疫反应性的抗-iNOS抗体的阳性细胞的数量和持续时间已被证明在再生芽。与50期幼虫不同，年龄较大的幼虫对断尾的反应程度更多地取决于个体蝌蚪，并且已观察到高百分比（70%-80%）的畸形尾巴。研究结果表明，非洲爪蟾尾巴再生效率的下降是由炎症反应和一氧化氮参与的差异驱动的。该分子是诱导的，并且是正常尾部再生所需的，而过量时，它可能与再生能力的进行性丧失有关。
The morphology and the immuno-distribution of the inducible isoform of nitric oxide synthase (iNOS) have been examined in regenerating tails from differently aged Xenopus laevis larvae. By comparing stage-50 and stage-55/56 tadpoles, various morphological aspects and immunoreactivity to anti-iNOS antibody in terms of the number and duration of positive cells have been demonstrated in the regenerating buds. Unlike in stage-50 larvae, the extent of responses to tail amputation in older larvae is more dependent on the individual tadpole and a high percentage (70%-80%) of malformed tails has been seen. The findings indicate that the decline in the efficiency of Xenopus tail regeneration is driven by differences in the inflammatory responses and in the involvement of nitric oxide. This molecule is induced and required for normal tail regeneration, whereas in excess, it is probably associated with progressive loss in the regeneration capability.