Inhibition of carbonic anhydrase by plasma of dogs and rabbits.
Inhibition of carbonic anhydrase by plasma of dogs and rabbits.
复制标题
狗和兔血浆对碳酸酐酶的抑制作用。
DOI：
10.1152/jappl.1986.60.1.191
复制
发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Hill,EP
中科院分区：
文献类型：
--
作者：
Hill,EP
通过Bowes-Davis技术测定犬红细胞、兔红细胞和犬肺组织匀浆稀释溶血液中的碳酸酐酶（CA）活性。在每种情况下，血浆（来自同一动物）抑制CA活性。对于1：16，700稀释的犬红细胞，CA催化CO2水合反应的速率是未催化速率的5.3 +/- 0.4倍，并且该活性的一半被0.44 +/-0.05%的血浆浓度抑制。相似的兔CA浓度受到1.02 +/-0.24%血浆浓度的抑制。即使在高血浆浓度下，来自犬肺组织匀浆的CA也仅被血浆部分抑制，这表明不同的同工酶，其中至少一种不被血浆抑制。结果表明，从人工灌注肺或组织学观察结果推断体内条件可能是无效的，应考虑至少在某些种属中血浆抑制的可能性。
Carbonic anhydrase (CA) activity was measured by the Bowes-Davis technique in diluted hemolysates of dog erythrocytes, rabbit erythrocytes, and dog lung tissue homogenates. Plasma (from the same animal) inhibited the CA activity in each case. For 1:16,700 dilution of dog erythrocytes, the CA catalyzed the CO2 hydration reaction by 5.3 +/- 0.4-fold above the uncatalyzed rate, and half that activity was inhibited by plasma concentrations of 0.44 +/- 0.05%. Similar rabbit CA concentrations were inhibited by plasma concentrations of 1.02 +/- 0.24%. CA from dog lung tissue homogenate is only partially inhibited by plasma even at high plasma concentrations, suggesting different isozymes, at least one of which is not inhibited by plasma. The results suggest that extrapolating from artificially perfused lungs or histological observations to in vivo conditions may not be valid, and the possibility of inhibition by plasma in at least some species should be considered.