Differential expression of the pituitary gonadotropin subunit genes during male rat sexual maturation: reciprocal relationship between hypothalamic pituitary adenylate cyclase-activating polypeptide and follicle-stimulating hormone beta expression.
Differential expression of the pituitary gonadotropin subunit genes during male rat sexual maturation: reciprocal relationship between hypothalamic pituitary adenylate cyclase-activating polypeptide and follicle-stimulating hormone beta expression.
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雄性大鼠性成熟过程中垂体促性腺激素亚基基因的差异表达：下丘脑垂体腺苷酸环化酶激活多肽与卵泡刺激素β表达之间的相互关系。
DOI：
10.1095/biolreprod.102.012757
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Winters,StephenJ
中科院分区：
文献类型：
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作者：
MooreJr,JosephP;Wilson,Lisa;Dalkin,AlanC;Winters,StephenJ
神经肽垂体腺苷酸环化酶激活多肽（PACAP）已被证明差异调节促性腺激素亚单位基因在培养的大鼠垂体细胞的表达。PACAP在下丘脑内表达，并且PACAP在门脉循环中的浓度比在全身循环中高2- 4倍。因此，PACAP是垂体功能的候选调节剂。在本研究中，我们研究了PACAP mRNA的表达室旁核（PVN）在成熟过程中（第20-60天）的雄性大鼠，并比较了这种表达的促性腺激素亚单位，卵泡抑素，垂体前叶内的GnRH受体mRNA的水平。FSH和LH的血清浓度证实了LH和FSH分泌发散的成熟模式。对促性腺激素亚基mRNA的北方分析显示，FSHβ表达与FSH分泌平行，而LHβ mRNA浓度在发育过程中不发生变化。垂体中GnRH受体的表达与FSHβ的表达平行。原位杂交显示PVN内PACAP mRNA的发育模式与FSHβ的发育模式相反。竞争性逆转录聚合酶链反应（RT-PCR）分析总垂体卵泡抑素mRNA没有显着变化，然而，半定量RT-PCR分析显示存在两个卵泡抑素mRNA的物种，其中之一，对应于卵泡抑素-288，发育调节。这些研究确定了成熟大鼠PVN PACAP和FSHβ基因表达之间的相互关系。我们认为PACAP可能通过调节卵泡抑素参与了雄性大鼠成熟过程中FSHβ表达的选择性调节。
The neuropeptide pituitary adenylate cyclase-activating polypeptide (PACAP) has been shown to differentially regulate the expression of the gonadotropin subunit genes in cultures of rat pituitary cells. PACAP is expressed within the hypothalamus, and concentrations of PACAP are 2- to 4-fold higher in the portal circulation than in the general circulation. Therefore, PACAP is a candidate regulator of pituitary function. In the present study, we examined the expression of PACAP mRNA within the paraventricular nucleus (PVN) during maturation (Days 20–60) in the male rat and compared this expression to the levels of the gonadotropin subunits, follistatin, and GnRH-receptor mRNAs within the anterior pituitary. Serum concentrations of FSH and LH confirm the established maturational pattern of divergent secretion of LH and FSH. Northern analysis of the gonadotropin subunit mRNAs revealed that FSHβ expression parallels FSH secretion whereas LHβ mRNA concentrations do not change during development. Expression of the GnRH receptor in the pituitary parallels that of FSHβ. In situ hybridization revealed a developmental pattern of PACAP mRNA within the PVN that is reciprocal to that of FSHβ. Competitive reverse transcriptase-polymerase chain reaction (RT-PCR) analysis of total pituitary follistatin mRNA revealed no significant changes; however, semiquantitative RT-PCR analyses revealed the presence of two follistatin mRNA species, one of which, corresponding to follistatin-288, was developmentally regulated. These studies identified a reciprocal relationship between PVN PACAP and FSHβ gene expression in maturing rats. We propose that PACAP contributes to the selective regulation of FSHβ expression during maturation in the male rat, perhaps via regulation of follistatin.