Direct Detection of Molecular Biorecognition by Dipole Sensing Mechanism
Direct Detection of Molecular Biorecognition by Dipole Sensing Mechanism
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DOI：
10.1021/ja809051p
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发表时间：
2009-04-08
影响因子：
15
通讯作者：
Yitzchaik, Shlomo
中科院分区：
文献类型：
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作者：
Goykhman, Ilya;Korbakov, Nina;Yitzchaik, Shlomo
这项工作研究了将分子识别事件转换为可测量的电位信号的可行性。首次证明乙酰胆碱（ACh）的生物识别可以转化为乙酰胆碱酯酶（AChE）的构象变化，从而引起表面电位的可测量变化。我们的结果表明，通过在浮栅场效应晶体管 (FG-FET) 上稀释组装 AChE 可以获得高灵敏度的 ACh 检测器。观察到 ACh (10(-2)-10(-9)M) 和抑制剂氨甲酰胆碱 CCh (10(-6)-10(-11)M) 的宽浓度范围响应。这些增强的灵敏度在理论上进行了建模，并通过设备对酶-底物识别事件导致的局部 pH 变化和分子偶极子变化的综合响应进行了解释。
This work investigates the feasibility of transducing molecular-recognition events into a measurable potentiometric signal. It is shown for the first time that biorecognition of acetylcholine (ACh) can be translated to conformational changes in the enzyme, acetylcholine-esterase (AChE), which in turn induces a measurable change in surface potential. Our results show that a highly sensitive detector for ACh can be obtained by the dilute assembly of AChE on a floating gate derived field effect transistor (FG-FET). A wide concentration range response is observed for ACh (10(-2)-10(-9)M) and for the inhibitor carbamylcholine CCh (10(-6)-10(-11)M). These enhanced sensitivities are modeled theoretically and explained by the combined response of the device to local pH changes and molecular dipole variations due to the enzyme-substrate recognition event.