Design and fabrication of a polyimide-based microelectrode array: Application in neural recording and repeatable electrolytic lesion in rat brain
Design and fabrication of a polyimide-based microelectrode array: Application in neural recording and repeatable electrolytic lesion in rat brain
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DOI：
10.1016/j.jneumeth.2009.05.010
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发表时间：
2009-08-30
影响因子：
3
通讯作者：
Lin, Si-Yue
中科院分区：
文献类型：
--
作者：
Chen, You-Yin;Lai, Hsin-Yi;Lin, Si-Yue
介绍了一种新型微电极阵列——国立交通大学探针的设计与测试。评价结果表明，该方法具有良好的生物相容性，在慢性神经记录过程中具有较高的信噪比（SNR：背景噪声与峰值平均峰幅的均方根），对电解损伤具有较高的可重用性。探针是一种柔性的聚酰亚胺基微电极阵列，具有长轴（14.9 mm长）和16个电极（5 μ m厚，16 μ m半径）；在啮齿类动物的慢性体内记录中检查了其性能。为了提高植入的精度，在聚酰亚胺层之间夹了一层金属抗冲击层来加强探针。电镀电极的三维结构产生了粗糙的纹理，增加了有效表面积。NCTU探针上电极的体外阻抗为2.4 +/- 0.52 M ω，频率为1 kHz。此外，植入NCTU探针的术后神经记录在清醒、行为正常的大鼠中进行了长达40天的记录。NCTU探针上的电极功能良好，具有高信噪比（范围：4-5）和可靠的体内阻抗(
The design and testing of a new microelectrode array, the NCTU (National Chiao Tung University) probe, was presented. Evaluation results showed it has good biocompatibility, high signal-to-noise ratio (SNR: the root mean square of background noise to the average peak-to-peak amplitude of spikes) during chronic neural recordings, and high reusability for electrolytic lesions. The probe was a flexible, polyimide-based microelectrode array with a long shaft (14.9 mm in length) and 16 electrodes (5 mu m-thick and 16 mu m in radius); its performance in chronic in vivo recordings was examined in rodents. To improve the precision of implantation, a metallic, impact-resistant layer was sandwiched between the polyimide layers to strengthen the probe. The three-dimensional (3D) structure of electrodes fabricated by electroplating produced rough textures that increased the effective surface area. The in vitro impedance of electrodes on the NCTU probe was 2.4 +/- 0.52 M Omega at 1 kHz. In addition, post-surgical neural recordings of implanted NCTU probes were conducted for up to 40 days in awake, normally behaving rats. The electrodes on the NCTU probe functioned well and had a high SNR (range: 4-5) with reliable in vivo impedance (