Characterization of TGF-β-regulated interleukin-8 expression in human prostate cancer cells
Characterization of TGF-β-regulated interleukin-8 expression in human prostate cancer cells
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DOI：
10.1002/pros.20424
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发表时间：
2006-06-15
期刊：
影响因子：
2.8
通讯作者：
Dong, Zhongyun
中科院分区：
文献类型：
--
作者：
Shan Lu;Dong, Zhongyun
背景白细胞介素（IL）-8和转化生长因子（TGF）-β 1在晚期前列腺癌中过表达本研究旨在探讨TGF-β 1对前列腺癌细胞IL-8表达的调节作用。通过实时逆转录PCR（RT-PCR）和蛋白质印迹法评估TGF-β受体表达。TGF-β 1调节的IL-8表达通过实时RT-PCR、酶联免疫吸附测定（ELISA）、核运行和IL-8启动子报告基因测定来确定。PC-3 MM 2细胞表达I型和II型TGF-β受体（T β RI和T β RII）。LNCaP细胞表达的T β RII水平显著降低。在PC-3 MM 2细胞和用T β RII工程化的LNCaP细胞（LNCaP-T β RII）中检测到IL-8的组成型表达。TGF-β 1以剂量和时间依赖性方式刺激IL-8的表达，这被放线菌酮（CHX）和放线菌素D（ActD）阻断。细胞核Run-on和IL-8荧光素酶报告基因检测显示TGF-β 1激活IL-8基因转录。TGF-β 1信号调节前列腺癌细胞中IL-8的表达，并可能导致人前列腺癌中IL-8的过度表达。
BACKGROUND. Interleukin (IL)-8 and transforming growth factor (TGF)-beta 1 are overexpressed in advanced prostate cancer. The purpose of this study was to investigate TGF-beta 1-regulated IL-8 expression in prostate cancer cells.METHODS. TGF-beta receptor expression was evaluated by real-time reverse-transcription PCR (RT-PCR) and Western blotting. TGF-beta 1-regulated IL-8 expression was determined by real-time RT-PCR, enzyme-linked immunoabsorbance assay (ELISA), nuclear run-on, and IL-8 promoter reporter assay.RESULTS. PC-3MM2 cells expressed type I and type II TGF-beta receptors (T beta RI and T beta RII). LNCaP cells expressed significantly lower level of T beta RII. Constitutive expression of IL-8 was detected in PC-3MM2 cells and LNCaP cells engineered with T beta RII (LNCaP-T beta RII). TGF-beta 1 stimulated IL-8 expression in dose- and time-dependent manners, which was blocked by cycloheximide (CHX) and actinomycin D (ActD). The nuclear run-on and IL-8 luciferase reporter assays show that TGF-beta 1 activated IL-8 gene transcription.CONCLUSIONS. TGF-beta 1 signaling regulates IL-8 expression in prostate cancer cells and may contribute to the overexpression of IL-8 in human prostate cancer.