Oral administration of a galactooligosaccharide preparation inhibits development of atopic dermatitis-like skin lesions in NC/Nga mice
Oral administration of a galactooligosaccharide preparation inhibits development of atopic dermatitis-like skin lesions in NC/Nga mice
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DOI：
10.3892/ijmm_00000349
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发表时间：
2010-03-01
影响因子：
5.4
通讯作者：
Hochi, Satomi
中科院分区：
文献类型：
--
作者：
Tanabe, Soichi;Hochi, Satomi
半乳糖低聚糖（GOS）存在于母乳中，经常添加到食物中以促进健康，研究人员在异位性皮炎（AD）的类人小鼠模型中评估了GOS的抗过敏作用。NC/Nga小鼠喂食5.5% GOS 8周，我们检测这种处理是否抑制了这些小鼠ad样皮肤病变的发展。与对照组相比，喂食GOS的小鼠皮炎症状明显减轻，抓挠频率减少，血清总免疫球蛋白E水平降低。8周实验结束时，取脾，用12-肉豆蔻酸13-乙酸佛波和离子霉素刺激脾细胞，分析细胞因子和趋化因子的产生。在gos喂养小鼠的脾细胞中观察到Th1细胞因子如干扰素γ水平升高。然而，Th2细胞因子如白细胞介素(IL)-13的水平没有变化。此外，GOS抑制炎症细胞因子如IL-1 β、IL-6、IL-17和肿瘤坏死因子的产生，但增强免疫调节性IL-10的产生。结果表明，GOS通过至少部分诱导IL-10的产生和抑制IL-17等参与皮肤炎症的细胞因子的产生，有效地阻断小鼠ad样皮肤病变。
Anti-allergic effects of galactooligosaccharide (GOS), which is found in breast milk and frequently added to food for promoting health, were evaluated in a human-like mouse model of atopic dermatitis (AD). NC/Nga mice were fed 5.5% GOS for 8 weeks, and we examined whether this treatment suppressed the development of AD-like skin lesions in these mice. Mice fed GOS exhibited significantly less symptoms of dermatitis, reduced scratching frequency, and lower levels of serum total immunoglobulin E compared to control. At the end of the 8-week-experimental period, spleens were removed, and the splenocytes were stimulated with phorbol 12-myristate 13-acetate and ionomycin, following which production of cytokines and a chemokine was analyzed. Elevated levels of Th1 cytokines such as interferon-gamma were observed in splenocytes from GOS-fed mice. However, the levels of Th2 cytokines such as interleukin (IL)-13 were unchanged. Furthermore, GOS inhibited the production of inflammatory cytokines such as IL-1 beta, IL-6, IL-17, and tumor necrosis factor-a but enhanced production of immunomodulatory IL-10. The results indicate that GOS effectively blocked AD-like skin lesions in the mice by at least partly inducing production of IL-10 and suppressing the production of cytokines such as IL-17, which are involved in skin inflammation.