Monte Carlo based method for fluorescence tomographic imaging with lifetime multiplexing using time gates.
Monte Carlo based method for fluorescence tomographic imaging with lifetime multiplexing using time gates.
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DOI：
10.1364/boe.2.000871
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发表时间：
2011-03-14
影响因子：
3.4
通讯作者：
Intes X
中科院分区：
文献类型：
--
作者：
Chen J;Venugopal V;Intes X
时间分辨荧光光学层析成像允许基于寿命对比度的多个荧光团的三维定位，同时提供用于提高分辨率的独特数据集。然而，为了采用完整的荧光时间测量，需要精确模拟弱漫射和多散射光子的光传播模型。在这篇文章中，我们推导出一个计算效率高的蒙特卡罗方法来计算时间选通的荧光雅可比矩阵的同时成像的两个荧光团的寿命对比度。基于蒙特卡罗的制剂在模拟具有寿命对比度的两种不同荧光团在肾脏中的摄取的合成鼠模型上进行验证。在实验上，使用填充有2.5nmol ICG和IRDye™800CW的毛细管分别包埋在模拟小鼠平均光学性质的扩散介质中来验证该方法。在一个反问题中结合多个时间门，允许使用所提出的配方同时重建多个荧光团，具有更高的分辨率和最小的串扰。
Time-resolved fluorescence optical tomography allows 3-dimensional localization of multiple fluorophores based on lifetime contrast while providing a unique data set for improved resolution. However, to employ the full fluorescence time measurements, a light propagation model that accurately simulates weakly diffused and multiple scattered photons is required. In this article, we derive a computationally efficient Monte Carlo based method to compute time-gated fluorescence Jacobians for the simultaneous imaging of two fluorophores with lifetime contrast. The Monte Carlo based formulation is validated on a synthetic murine model simulating the uptake in the kidneys of two distinct fluorophores with lifetime contrast. Experimentally, the method is validated using capillaries filled with 2.5nmol of ICG and IRDye™800CW respectively embedded in a diffuse media mimicking the average optical properties of mice. Combining multiple time gates in one inverse problem allows the simultaneous reconstruction of multiple fluorophores with increased resolution and minimal crosstalk using the proposed formulation.