THE BETA-LIKE-GLOBIN GENE DOMAIN IN HUMAN ERYTHROID-CELLS
THE BETA-LIKE-GLOBIN GENE DOMAIN IN HUMAN ERYTHROID-CELLS
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DOI：
10.1073/pnas.82.19.6384
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发表时间：
1985-01-01
影响因子：
11.1
通讯作者：
LONDON, IM
中科院分区：
文献类型：
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作者：
TUAN, D;SOLOMON, W;LONDON, IM
我们已经绘制了人“β-DNA酶I超敏感位点”中主要和次要DNA酶I超敏感位点的分布图。类珠蛋白基因结构域。次要的DNA酶I-超敏感性位点定位在每个β-DNA酶的5“末端附近。类珠蛋白基因。它们的存在与直接下游的β-受体的转录特异性相关。类珠蛋白基因。主要的DNA酶I-超敏感位点映射在似乎是人β-DNA酶的5“和3”边界区域的位置。类珠蛋白基因结构域，一个估计跨越至少90个DNA酶的区域。这些主要位点存在于各种红系细胞中，这些红系细胞主要表达胚胎、胎儿或成人的β-类似珠蛋白基因，并且似乎参与定义活性β-珠蛋白基因。红系细胞中的类珠蛋白基因结构域。当与测序数据相关联时，5“边界区域中的四个主要DNase I超敏感位点显示位于含有增强子核心样序列和交替的嘌呤和嘧啶碱基的DNA区域中。
We have mapped the distribution of the major and minor DNase I-hypersensitive sites in the human ".beta.-like-globin" gene domain. The minor DNase I-hypersensitive sites map close to the 5'' end of each of the .beta.-like-globin genes. Their presence is specifically associated with the transcription of the immediate downstream .beta.-like-globin genes. The major DNase I-hypersensitive sites map in what appear to be the 5'' and 3'' boundary areas of the human .beta.-like-globin gene domain, a region estimated to span at least 90 kilobases of DNA. These major sites are present in various erythroid cells, which express predominantly either the embryonic, the fetal, or the adult .beta.-like-globin genes, and seem to be involved in defining the active .beta.-like-globin gene domain in cells of erythroid lineage. The four major DNase I-hypersensitive sites in the 5'' boundary area, when correlated with sequencing data, are shown to be located in DNA regions containing enhancer core-like sequences and alternating purine and pyrimidine bases.