A NEW TYPE OF ULTRASENSITIVE BIOLUMINOGENIC ENZYME SUBSTRATES .1. ENZYME SUBSTRATES WITH D-LUCIFERIN AS LEAVING GROUP
A NEW TYPE OF ULTRASENSITIVE BIOLUMINOGENIC ENZYME SUBSTRATES .1. ENZYME SUBSTRATES WITH D-LUCIFERIN AS LEAVING GROUP
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DOI：
10.1515/bchm3.1988.369.1.407
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发表时间：
1988-05-01
期刊：
影响因子：
--
通讯作者：
GEIGER, R
中科院分区：
文献类型：
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作者：
MISKA, W;GEIGER, R
D-荧光素、D-荧光素甲酯、D-荧光素O-硫酸盐、D-荧光素O-磷酸、D-荧光素-L-Nα-精氨酸和D-荧光素-L-苯丙氨酸的衍生物被用作羧酸酯酶、芳基硫酸酯酶、碱性磷酸酶和羧肽酶A、B和N的高灵敏底物。测定了D-荧光素甲酯和羧酸酯酶、D-荧光素O-硫酸酯和芳基硫酸酯酶、D-荧光素O-磷酸和碱性磷酸酶、D-荧光素-L-苯丙氨酸和羧肽酶A、羧肽酶B和N以及D-荧光素-L-Nα-精氨酸的动力学常数。所有化合物都被证明是各自酶的高度敏感的底物，允许每次检测酶的极限在10到500 fg之间。
Derivatives of D-luciferin, D-luciferin methyl ester, D-luciferin O-sulfate, D-luciferin O-phosphate, D-luciferyl-L-N.alpha.-arginine and D-luciferyl-L-phenylalaine were used as highly sensitive substrates for carboxylic esterase, arylsulfatase, alkaline phosphatase and carboxypeptidases A, B and N. Enzymatic cleavage of the compounds by enzymes leading to the release of D-luciferin was demonstrated. Kinetic constants have been determined for D-luciferin methyl ester and carboxylic esterase, for D-luciferin O-sulfate and arylsulfatase, for D-luciferin O-phosphate and alkaline phosphatase, for D-luciferyl-L-phenylalanine and carboxypeptidase A, and for carboxypeptidases B and N and D-luciferyl-L-N.alpha.-arginine. All compounds proved to be highly sensitive substrates for the respective enzymes, permitting a limit of detection for enzymes between 10 and 500 fg per assay.