Arabidopsis adaptor protein 1G is critical for pollen development
Arabidopsis adaptor protein 1G is critical for pollen development
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拟南芥衔接蛋白1G对于花粉发育至关重要
DOI：
10.1111/jipb.12556
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发表时间：
2017-09-01
影响因子：
11.4
通讯作者：
Zhang, Yan
中科院分区：
文献类型：
--
作者：
Feng, Chong;Wang, Jia-Gang;Zhang, Yan
花粉发育是被子植物有性繁殖的先决条件，在有性繁殖过程中涉及多种细胞活动。花粉发育伴随着液泡分裂和融合的动态重塑，液泡分裂和融合的破坏往往会影响花粉的活力。我们以前报道过，在花粉管接受过程中，适配器蛋白1的Y亚基(AP1G)介导了助细胞的退化。在这里，我们证明了AP1G对于花粉发育是必不可少的。由于花粉发育缺陷，AP1G功能丧失导致雄配子体死亡。通过超微结构分析和荧光标记，我们证明AP1G功能丧失影响了花粉发育过程中动态的液泡重塑，并削弱了花粉的空泡酸化。本文的结果支持液泡在配子体花粉发育中的关键作用。
Pollen development is a pre-requisite for sexual reproduction of angiosperms, during which various cellular activities are involved. Pollen development accompanies dynamic remodeling of vacuoles through fission and fusion, disruption of which often compromises pollen viability. We previously reported that the Y subunit of adaptor protein 1 (AP1G) mediates synergid degeneration during pollen tube reception. Here, we demonstrate that AP1G is essential for pollen development. AP1G loss-of-function resulted in male gametophytic lethality due to defective pollen development. By ultrastructural analysis and fluorescence labeling, we demonstrate that AP1G loss-of-function compromised dynamic vacuolar remodeling during pollen development and impaired vacuolar acidification of pollen. Results presented here support a key role of vacuoles in gametophytic pollen development.