A Molecular Signature of Two Long Non-Coding RNAs in Peripheral Blood Predicts Acute Renal Allograft Rejection
A Molecular Signature of Two Long Non-Coding RNAs in Peripheral Blood Predicts Acute Renal Allograft Rejection
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外周血中两个长非编码 RNA 的分子特征预测急性肾同种异体移植排斥
DOI：
10.1159/000485451
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发表时间：
2017-01-01
影响因子：
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通讯作者：
Jia, Rui-Peng
中科院分区：
文献类型：
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作者：
Ge, Yu-Zheng;Xu, Tao;Jia, Rui-Peng
背景/目的：急性排斥反应（AR）是肾移植术后的主要并发症，目前尚无公认的非侵入性生物标志物。本研究旨在探讨长链非编码RNA（lncRNA）在肾移植受者外周血（PB）中的表达谱及其潜在的诊断价值。方法：分析了来自儿科和成人肾移植（PRTx和ARTx）队列的150份PB样本的全基因组lncRNA表达谱。采用受试者工作特征曲线（AUC）和95%置信区间（CI）确定差异表达lncRNA的诊断性能。最后，用逻辑回归模型构建风险评分。结果：在PRTx组中共发现162个lncRNA差异表达，在ARTx组中共发现163个lncRNA差异表达。在这些鉴定的lncRNA中，23个相应地在两个队列中去调节，并且可以区分AR接受者与没有AR的那些。最后，生成了具有两种最显著lncRNA（AF 264622和AB 209021）的风险评分，并且在PRTx（AUC：0.829，95%CI：0.735-0.922）和ARTx队列（AUC：0.889，95%CI：0.817-0.960）中均表现出优异的诊断性能。结论：PB中两种lncRNA的分子特征可以作为诊断儿童和成人肾移植受者AR的新型非侵入性生物标志物。
Background/Aims: Acute rejection (AR) is a major complication post renal transplantation, with no widely-accepted non-invasive biomarker. This study aimed to explore the expression profiles of long non-coding RNAs (lncRNAs) in the peripheral blood (PB) of renal transplant recipients and their potential diagnostic values. Methods: The genome-wide lncRNA expression profiles were analyzed in 150 PB samples from pediatric and adult renal transplant (PRTx and ARTx) cohorts. The diagnostic performance of differentially expressed lncRNA was determined using receiver operator characteristic curve, with area under the curve (AUC) and 95% confidential interval (CI). Finally, a risk score was constructed with logistical regression model. Results: A total of 162 lncRNAs were found differentially expressed in PRTx cohort, while 163 in ARTx cohort. Among these identified lncRNAs, 23 deregulated accordingly in both cohorts, and could distinguish AR recipients from those without AR. Finally, a risk score with two most significant lncRNAs (AF264622 and AB209021) was generated and exhibited excellent diagnostic performance in both PRTx (AUC:0.829, 95% CI:0.735-0.922) and ARTx cohorts (AUC: 0.889, 95% CI: 0.817-0.960). Conclusion: A molecular signature of two lncRNAs in PB could serve as a novel non-invasive biomarker for the diagnosis of AR in both pediatric and adult renal transplant recipients.