Mutation of FdC2 gene encoding a ferredoxin-like protein with C-terminal extension causes yellow-green leaf phenotype in rice
Mutation of FdC2 gene encoding a ferredoxin-like protein with C-terminal extension causes yellow-green leaf phenotype in rice
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编码 C 端延伸的铁氧还蛋白样蛋白的 FdC2 基因突变导致水稻黄绿叶表型
DOI：
10.1016/j.plantsci.2015.06.010
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发表时间：
2015-09-01
期刊：
影响因子：
5.2
通讯作者：
Wang, Pingrong
中科院分区：
文献类型：
--
作者：
Li, Chunmei;Hu, Yan;Wang, Pingrong
铁氧还蛋白（Ferredoxins，Fds）是一种小分子铁硫蛋白，在多种代谢反应中介导电子传递。除Fds外，还有一类Fd样蛋白，命名为FdC，其具有Fds的保守元件，但含有显著的C-末端延伸。到目前为止，在高等植物中只发现了两个拟南芥FdC基因，因此FdC蛋白的功能仍不清楚。在本研究中，我们从水稻（Oryza sativa）中分离到一个黄绿叶突变体501ys。突变体表现出黄绿叶表型和叶绿素水平降低。501ys的表型是由水稻3号染色体上的一个基因突变引起的。对该突变体进行图位克隆，得到OsFdC2基因（LOC_Os03g48040），该基因与拟南芥FdC2基因（AT1G32550）具有较高的同源性。OsFdC2在叶片中表达量最高，其编码蛋白定位于叶绿体。在501ys突变体中，在基因的DNA序列中检测到错义突变，导致编码蛋白的氨基酸改变。通过引入野生型基因拯救了突变表型。因此，我们通过图位克隆的方法成功地鉴定了FdC2基因，并证明该基因的突变导致了水稻的黄绿叶表型。(C)2015爱思唯尔爱尔兰有限公司版权所有。
Ferredoxins (Fds) are small iron-sulfur proteins that mediate electron transfer in a wide range of metabolic reactions. Besides Fds, there is a type of Fd-like proteins designated as FdC, which have conserved elements of Fds, but contain a significant C-terminal extension. So far, only two FdC genes of Arabidopsis (Arabidopsis thaliana) have been identified in higher plants and thus the functions of FdC proteins remain largely unknown. In this study, we isolated a yellow-green leaf mutant, 501ys, in rice (Oryza sativa). The mutant exhibited yellow-green leaf phenotype and reduced chlorophyll level. The phenotype of 501ys was caused by mutation of a gene on rice chromosome 3. Map-based cloning of this mutant resulted in identification of OsFdC2 gene (LOC_Os03g48040) showing high identity with Arabidopsis FdC2 gene (AT1G32550). OsFdC2 was expressed most abundantly in leaves and its encoded protein was targeted to the chloroplast. In 501ys mutant, a missense mutation was detected in DNA sequence of the gene, resulting in an amino acid change in the encoded protein. The mutant phenotype was rescued by introduction of the wild-type gene. Therefore, we successfully identified FdC2 gene via map-based cloning approach, and demonstrated that mutation of this gene caused yellow-green leaf phenotype in rice. (C) 2015 Elsevier Ireland Ltd. All rights reserved.