Development of a pan-neuronal genetic driver in Aedes aegypti mosquitoes.
Development of a pan-neuronal genetic driver in Aedes aegypti mosquitoes.
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埃及伊蚊泛神经元遗传驱动的开发。
DOI：
10.1016/j.crmeth.2021.100042
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
McBride,CarolynS
中科院分区：
文献类型：
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作者：
Zhao,Zhilei;Tian,David;McBride,CarolynS
埃及伊蚊的神经遗传学工具的最新发展，开始阐明这种行为的神经基础，使这种物种成为人类疾病的主要载体。然而，我们仍然缺乏一个泛神经元表达驱动程序-一个关键的工具，提供遗传访问所有神经元。在这里，我们描述了我们通过CRISPR/Cas9介导的报告基因敲入广泛表达的神经基因来填补这一空白的努力，并报告了两种菌株的产生，aSyt 1：GCaMP 6s菌株表达突触定位的GCaMP和abrp-T2 A-QF 2 wdriver菌株，可用于通过Q二元系统驱动和扩增任何效应子的表达。这两种操作都广泛而统一地标记了神经系统，对行为只有适度的影响。我们希望这些菌株能促进埃及伊蚊的神经生物学研究，并记录成功和失败的操作，作为其他非模式物种类似工具开发的路线图。
The recent development of neurogenetic tools inAedes aegyptimosquitoes is beginning to shed light on the neural basis of behaviors that make this species a major vector of human disease. However, we still lack a pan-neuronal expression driver—a key tool that provides genetic access to all neurons. Here, we describe our efforts to fill this gap via CRISPR/Cas9-mediated knock-in of reporters to broadly expressed neural genes and report on the generation of two strains, aSyt1:GCaMP6sstrain that expresses synaptically localized GCaMP and abrp-T2A-QF2wdriver strain that can be used to drive and amplify expression of any effector via the Q binary system. Both manipulations broadly and uniformly label the nervous system with only modest effects on behavior. We expect these strains to facilitate neurobiological research inAe.aegyptimosquitoes and document both successful and failed manipulations as a roadmap for similar tool development in other non-model species.