Comparative molecular species delimitation in the charismatic Nawab butterflies (Nymphalidae, Charaxinae, Polyura).
Comparative molecular species delimitation in the charismatic Nawab butterflies (Nymphalidae, Charaxinae, Polyura).
复制标题
DOI：
10.1016/j.ympev.2015.05.015
复制
发表时间：
2015-10
影响因子：
4.1
通讯作者：
Emmanuel F. A. Toussaint;J. Morinière;Chris J. Müller;Krushnamegh Kunte;Bernard Turlin;A. Hausmann;M. Balke
中科院分区：
文献类型：
--
作者：
Emmanuel F. A. Toussaint;J. Morinière;Chris J. Müller;Krushnamegh Kunte;Bernard Turlin;A. Hausmann;M. Balke
魅力十足的热带PolyuraNawab蝴蝶分布在印度/澳大利亚群岛的12个生物多样性热点地区。在这项研究中，我们测试了一系列的物种界定方法，并将结果与现有的基于形态学的分类法进行了比较。我们测序了两个线粒体和两个核基因片段，以重建系统发育关系内Polyura使用贝叶斯推断和最大似然法。基于这一系统发育框架，我们使用最近引入的bGMYC，BPP和PTP方法来研究物种边界。根据上述结果，我们描述了两个新种：波氏多尾虫PolyurapaulettaeToussaintsp. n.和Polyura smilesi Toussaint sp. n.，提出了一个异名，五个种群被提升到种的地位。大多数新发现的物种是单岛特有种，可能是由于印度-澳大利亚群岛最近高度复杂的地质历史造成的。令人惊讶的是，我们还发现两个新认识的物种在Indomalayan地区，额外的生物或非生物因素促进了物种形成。物种划界方法在很大程度上是一致的，并成功地交叉验证了大多数现存的形态物种。PTP和BPP似乎产生更一致和强大的估计物种边界的形态特征，而bGMYC提供对比的结果，这取决于不同的基因树考虑。我们的研究结果表明，比较方法的效率，使用分子物种划界方法的经验数据。它们还为调查不太知名的群体铺平了道路，以揭示物种丰富度的模式，并对地球上隐藏的、因此未被欣赏的多样性进行编目。
The charismatic tropicalPolyuraNawab butterflies are distributed across twelve biodiversity hotspots in the Indomalayan/Australasian archipelago. In this study, we tested an array of species delimitation methods and compared the results to existing morphology-based taxonomy. We sequenced two mitochondrial and two nuclear gene fragments to reconstruct phylogenetic relationships withinPolyurausing both Bayesian inference and maximum likelihood. Based on this phylogenetic framework, we used the recently introduced bGMYC, BPP and PTP methods to investigate species boundaries. Based on our results, we describe two new speciesPolyura paulettaeToussaint sp. n. andPolyura smilesiToussaint sp. n., propose one synonym, and five populations are raised to species status. Most of the newly recognized species are single-island endemics likely resulting from the recent highly complex geological history of the Indomalayan–Australasian archipelago. Surprisingly, we also find two newly recognized species in the Indomalayan region where additional biotic or abiotic factors have fostered speciation. Species delimitation methods were largely congruent and succeeded to cross-validate most extant morphological species. PTP and BPP seem to yield more consistent and robust estimations of species boundaries with respect to morphological characters while bGMYC delivered contrasting results depending on the different gene trees considered. Our findings demonstrate the efficiency of comparative approaches using molecular species delimitation methods on empirical data. They also pave the way for the investigation of less well-known groups to unveil patterns of species richness and catalogue Earth’s concealed, therefore unappreciated diversity.