Expression of the potent vasoconstrictor endothelin in the human central nervous system.
Expression of the potent vasoconstrictor endothelin in the human central nervous system.
复制标题
强效血管收缩剂内皮素在人类中枢神经系统中的表达。
DOI：
10.1172/jci114677
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Quertermous,T
中科院分区：
文献类型：
--
作者：
Lee,ME;delaMonte,SM;Ng,SC;Bloch,KD;Quertermous,T
内皮素是一种有效的血管收缩肽，最初被认为是内皮细胞的产物。为了研究内皮素作为一种神经肽的潜在作用，我们研究了它在人类中枢神经系统中的分布。RNA印迹杂交提供了内皮素基因转录在脑的各种功能区域的证据。原位杂交证实了内皮素转录的广泛模式，并表明含有内皮素mRNA的细胞密度最高的是下丘脑。该技术将内皮素转录定位到神经系统和血管内皮细胞。免疫细胞化学研究检测到内皮素在神经元中的免疫反应性，提供了在这种细胞类型中合成肽的证据，并证实内皮素是一种神经肽。虽然内皮素在下丘脑的显著表达可能表明该肽具有中枢血管调节作用，但内皮素在大脑其他区域神经元中的广泛分布表明其在神经系统功能调节中具有更根本的作用。图片
Endothelin is a potent vasoconstrictive peptide initially characterized as a product of endothelial cells. To examine the potential role of endothelin as a neuropeptide, we studied its distribution in the human central nervous system. RNA blot hybridization provided evidence of endothelin gene transcription in a variety of functional regions of the brain. In situ hybridization confirmed the widespread pattern of endothelin transcription and indicated that the highest density of cells containing endothelin mRNA is in the hypothalamus. This technique localized endothelin transcription to cells of the nervous system as well as the vascular endothelium. Immunocytochemical studies detected endothelin immunoreactivity in neurons, providing evidence of the synthesis of the peptide in this cell type and confirming that endothelin is a neuropeptide. Although the prominent expression of endothelin in the hypothalamus may indicate a central vasoregulatory role for the peptide, the widespread distribution of endothelin in neurons in other areas of the brain implies a more fundamental role in the regulation of nervous system function.Images