Electrochemiluminescent aptasensor for thrombin using nitrogen-doped graphene quantum dots
Electrochemiluminescent aptasensor for thrombin using nitrogen-doped graphene quantum dots
复制标题
使用氮掺杂石墨烯量子点的电化学发光适体传感器用于凝血酶
DOI：
10.1007/s00604-018-2942-z
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发表时间：
2018
影响因子：
5.7
通讯作者：
Shiguo Sun
中科院分区：
文献类型：
--
作者：
YasaminNasiri Khonsari;Shiguo Sun
介绍了一种基于电化学发光(ECL)适体的凝血酶测定方法。将三维掺氮氧化石墨烯(3D-NGO)放置在玻碳电极(GCE)上，获得了具有良好导电性的电极表面，并作为ECL的强发射极。通过电沉积的方法在修饰电极上进一步涂覆了壳聚糖。最后，将氨基修饰适体固定在修饰后的GCE上。凝血酶和适体之间的相互作用导致ECL降低。该方法在1 fM~1 nM凝血酶浓度范围内具有良好的线性关系，检出限为0.25fM(S/氮比为3)。方法用于人血浆中凝血酶的测定，加标回收率为94%~105%(RSD=3.6%)。在荧光发射的电位和波长(波长：445 nm；电位：0~-2V)下记录校正曲线。
An electrochemiluminescent (ECL) aptamer based method is described for the determination of thrombin. Three-dimensional nitrogen-doped graphene oxide (3D-NGO) was placed on a glassy carbon electrode (GCE) to provide an electrode surface that displays excellent electrical conductivity and acts as a strong emitter of ECL. The modified electrode was further coated with chitosan via electrodeposition. Finally, the amino-modified aptamer was immobilized on the modified GCE. The interaction between thrombin and aptamer results in a decrease in ECL. The assay has a linear response in the 1 fM to 1 nM thrombin concentration range and a 0.25 fM lower detection limit (at an S/N ratio of 3). The method was applied to the determination of thrombin in spiked human plasma samples, and recoveries ranged between 94 and 105% (with RSDs of <3.6%). The calibration plot was recorded at potential and wavelength of fluorescence emission (wavelength: 445 nm; potential: 0 to -2 V).