A sensor tip based on carbon nanotube-ink printed electrode for the dengue virus NS1 protein
A sensor tip based on carbon nanotube-ink printed electrode for the dengue virus NS1 protein
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DOI：
10.1016/j.bios.2012.12.033
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发表时间：
2013-06-15
影响因子：
12.6
通讯作者：
Dutra, Rosa F.
中科院分区：
文献类型：
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作者：
Dias, Ana Carolina M. S.;Gomes-Filho, Sergio L. R.;Dutra, Rosa F.
研制了一种基于碳纳米管-丝网印刷电极的登革病毒非结构蛋白1(NS1)免疫传感器。将含有羧化碳纳米管的均匀混合物分散在碳墨水中，制成丝网印刷工作电极。抗NS1抗体通过乙二胺膜策略共价连接到CNT-SPE上。过氧化氢与抗NS1抗体偶联的过氧化氢酶(HRP)在-0.5V与Ag/AgCl反应产生电流响应。获得了良好的检测下限(约12ngmL(-1))和85.59mA mm(-1)cm(-2)的灵敏度，允许根据临床范围进行登革热诊断。使用添加的血清样本成功地评估了基质效应以及分析的性能，在结果中获得了良好的回收率。将碳纳米管引入碳墨水中，提高了CNT-SPE免疫传感器的重现性和灵敏度。这一点护理方法在疫情中具有很大的潜在使用价值，并可促进对处于登革热病毒急性期的患者进行早期筛查。(C)2012爱思唯尔B.V.保留所有权利。
An immunosensor for the non-structural protein 1 (NS1) of the dengue virus based on carbon nanotube-screen printed electrodes (CNT-SPE) was successfully developed. A homogeneous mixture containing carboxylated carbon nanotubes was dispersed in carbon ink to prepare a screen printed working electrode. Anti-NS1 antibodies were covalently linked to CNT-SPE by an ethylenediamine film strategy. Amperometrical responses were generated at -0.5 V vs. Ag/AgCl by hydrogen peroxide reaction with peroxidase (HRP) conjugated to the anti-NS1. An excellent detection limit (in the order of 12 ng mL(-1)) and a sensitivity of 85.59 mu A mM(-1) cm(-2) were achieved permitting dengue diagnostic according to the clinical range required. The matrix effect, as well as the performance of the assays, was successfully evaluated using spiked blood serum sample obtaining excellent recovery values in the results. Carbon nanotubes incorporated to the carbon ink improved the reproducibility and sensitivity of the CNT-SPE immunosensor. This point-of-care approach represents a great potential value for use in epidemic situations and can facilitate the early screening of patients in acute phase of dengue virus. (c) 2012 Elsevier B.V. All rights reserved.