POST-TRANSCRIPTIONAL MODIFICATIONS OF OAT COLEOPTILE RIBONUCLEIC-ACIDS - 5'-TERMINAL CAPPING AND METHYLATION OF INTERNAL NUCLEOSIDES IN POLY(A)-RICH RNA
POST-TRANSCRIPTIONAL MODIFICATIONS OF OAT COLEOPTILE RIBONUCLEIC-ACIDS - 5'-TERMINAL CAPPING AND METHYLATION OF INTERNAL NUCLEOSIDES IN POLY(A)-RICH RNA
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DOI：
10.1111/j.1432-1033.1980.tb04425.x
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发表时间：
1980-01-01
期刊：
影响因子：
--
通讯作者：
CLINE, MG
中科院分区：
文献类型：
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作者：
HAUGLAND, RA;CLINE, MG
有证据表明燕麦胚芽鞘组织中富含聚(A)的RNA中存在5‘-末端封帽结构。与其他真核生物的RNA相似，燕麦胚芽鞘RNA分子的5‘’末端由7-甲基鸟苷通过2个焦磷酸键中的3个磷酸基团与相邻的(倒数第二个)核苷连接而成。倒数第二个核苷主要含有嘌呤碱基，但也可检测到少量的嘧啶(胞苷)。还检测到一些单磷酸化的5‘-末端，但它们似乎是由于RNA降解而发生的。除了5‘’帽，燕麦RNA分子还被转录后修饰，内部腺苷的N6-甲基化频率很低。
Evidence is presented for the occurrence of 5''-terminal capping structure in poly(A)-rich RNA from oat coleoptile tissue. Similar to the cap structures in mRNA from other eukaryotic organisms, the 5'' terminus of these oat coleoptile RNA molecules consists of 7-methylguanosine joined 5'' to 5'' with the adjacent (penultimate) nucleoside by means of 3 phosphate groups in 2 pyrophosphate linkages. The penultimate nucleoside contains primarily purine bases, but small amounts of pyrimidines (cytidine) are also detectable. Some monophosphorylated 5''-termini were also detected, but they appear to occur as a result of RNA degradation. In addition to the 5'' cap, oat RNA molecules are also post-transcriptionally modified with a low frequency of N6-methylations of internal adenosines.