Hollow mesoporous zirconia delivery system for biomineralization precursors
Hollow mesoporous zirconia delivery system for biomineralization precursors
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用于生物矿化前体的中空介孔氧化锆输送系统。
DOI：
10.1016/j.actbio.2017.11.049
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发表时间：
2018-02-01
影响因子：
9.7
通讯作者：
Niu, Li-na
中科院分区：
文献类型：
--
作者：
Huang, Xue-qing;Yang, Hong-ye;Niu, Li-na
基于纳米载体递送系统和支架相结合的策略提供了具有多功能的骨组织工程支架。利用生物相容性陶瓷氧化锆制备中空介孔纳米胶囊，用于负载、储存和缓释新型多胺稳定的液体无定形磷酸钙前体相(PAH-ACP)，用于胶原的生物矿化和骨髓基质细胞的成骨诱导。负载仿生前驱体的中空介孔氧化锆纳米粒具有对pH敏感的释放能力和良好的生物相容性。从负载的hmZrO(2)中释放的PAH-ACP仍然保持了渗透和矿化胶原纤维的能力，并表现出骨诱导活性。将PAH-ACP@hmZrO(2)补充剂和干细胞混合的胶原支架可能是一种有前途的骨组织工程工具。意义纳米技术的出现促进了基于纳米载体递送系统和支架相结合的骨组织工程策略的发展，这些策略提供了独特的优势，包括生物活性物质的持续释放和保护，特定部位的药理作用和减少副作用。本论文合成了具有pH敏感能力的中空介孔氧化锆(hmZrO(2))纳米胶囊，用于负载、储存和缓释一种新型的多胺稳定的ACP前驱体相(PAH-ACP)。负载的纳米胶囊具有良好的生物相容性，对胶原的生物矿化和骨髓基质细胞的成骨具有生物活性。我们的研究结果可能为设计骨组织工程“鸡尾疗法”提供一个有希望的工具，该疗法包括以生物矿化前体负载hmZrO(2)补充剂和干细胞为种子的支架。(C)2017 Acta Materialia Inc.由Elsevier Ltd.出版。保留所有权利。
Strategies based on the combination of nanocarrier delivery systems and scaffolds provide bone tissue engineering scaffolds with multifunctional capability. Zirconia, a biocompatible ceramic commonly used in orthopedic and dental implants, was used to synthesize hollow mesoporous nanocapsules for loading, storage and sustained release of a novel polyamine-stabilized liquid precursor phase of amorphous calcium phosphate (PAH-ACP) for collagen biomineralization and bone marrow stromal cells osteoinduction. Hollow mesoporous zirconia (hmZrO(2)) nanocapsules loaded with biomimetic precursors exhibited pH-sensitive release capability and good biocompatibility. The PAH-ACP released from loaded hmZrO(2) still retained the ability to infiltrate and mineralize collagen fibrils as well as exhibited osteoinductivity. A collagen scaffold blended with PAH-ACP@hmZrO(2) supplement and stem cells may be a promising tool for bone tissue engineering.Statement of SignificanceThe advent of nanotechnology has catalyzed the development of bone tissue engineering strategies based on the combination of nanocarrier delivery systems and scaffolds, which provide distinct advantages, including the possibilities of sustained release and protection of the bioactive agents, site-specific pharmacological effects and reduction of side effects. Herein, hollow mesoporous zirconia (hmZrO(2)) nanocapsules with pH-sensitive capacity were synthesized for loading, storage and sustained release of a novel polyamine-stabilized liquid precursor phase of ACP (PAH-ACP). The loaded nanocapsules show good biocompatibility and demonstrate bioactivities for collagen biomineralization and bone marrow stromal cells osteoinduction. Our results may offer a promising tool for designing bone tissue engineering "cock-tail therapy" involving seeding scaffolds with biomineralization precursors loaded hmZrO(2) supplement and stem cells. (C) 2017 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.