The abnormal eosinophils are part of the leukemic cell population in acute myelomonocytic leukemia with abnormal eosinophils (AML M4Eo) and carry the pericentric inversion 16: A combination of May-Grunwald-Giemsa staining and fluorescence in situ hybridization
The abnormal eosinophils are part of the leukemic cell population in acute myelomonocytic leukemia with abnormal eosinophils (AML M4Eo) and carry the pericentric inversion 16: A combination of May-Grunwald-Giemsa staining and fluorescence in situ hybridization
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DOI：
10.1182/blood.v87.6.2459.bloodjournal8762459
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发表时间：
1996-03-15
期刊：
影响因子：
20.3
通讯作者：
Schlegelberger, B
中科院分区：
文献类型：
--
作者：
Haferlach, T;Winkemann, M;Schlegelberger, B
法国-美国-英国亚型急性髓单核细胞白血病伴异常嗜酸性粒细胞（FAB AML M4 Eo）伴16号染色体臂间倒位，在细胞形态学上由髓单核母细胞原始细胞群和异常嗜酸性粒细胞定义。到目前为止，这些异常嗜酸性粒细胞是恶性克隆的一部分还是一种附带现象仍然是一个悬而未决的问题。我们分析了5例伴有inv（16）的AML M4 Eo，并结合May-Grunwald-Giemsa染色和酵母人工染色体克隆854 E2的荧光原位杂交，该克隆跨越16 p上的inv（le）断点。在inv（16）的情况下，在中期扩散和间期细胞中均可观察到三个杂交信号，而不是正常的两个杂交信号。通过这种方法，我们能够在异常嗜酸性粒细胞中显示倒位16，因此，将它们鉴定为白血病细胞群的一部分。(C)1996年，美国血液学会。
The French-American-British subtype acute myelomonocytic leukemia with abnormal eosinophils (FAB AML M4Eo) with pericentric inversion of chromosome 16 is cytomorphologically defined by a myelomonoblastic blast population and abnormal eosinophils. Until now, it remained an open question whether these abnormal eosinophils are part of the malignant clone or an epiphenomenon. We analyzed five cases of AML M4Eo with inv(16) and combined May-Grunwald-Giemsa staining with fluorescence in situ hybridization using yeast artificial chromosome clone 854E2, which spans the inv(le) breakpoint on 16p. In the case of inv(16), three instead of the normal two hybridization signals can be observed both on metaphase spreads and in interphase cells. With this approach, we were able to show inversion 16 in abnormal eosinophils and, therefore, identified them as a part of the leukemic cell population. (C) 1996 by The American Society of Hematology.