Surface Modification of Porous Titanium Granules for Improving Bioactivity
Surface Modification of Porous Titanium Granules for Improving Bioactivity
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DOI：
10.11607/jomi.5246
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发表时间：
2016-11-01
影响因子：
2
通讯作者：
Faghihi, Shahab
中科院分区：
文献类型：
--
作者：
Karaji, Zahra Gorgin;Houshmand, Behzad;Faghihi, Shahab
目的：高多孔钛颗粒是目前常用的骨替代材料和骨组织增强材料。然而，它们的骨结合能力较弱。本研究的目的是在不规则形状的钛颗粒上建立纳米结构的表面氧化层，以提高其生物活性。这可以通过优化电化学阳极氧化（阳极氧化）和热处理工艺来实现。材料与方法：在以乙二醇为基础的电解液中，在60v的最佳条件下阳极氧化3小时。随后将氧化颗粒在450℃下退火1小时。利用扫描电子显微镜（SEM）、能谱仪（EDS）和x射线衍射仪（XRD）对颗粒的表面结构和形貌进行表征。通过将样品浸泡在模拟体液（SBF）中1,2,3周来评估样品的体外生物活性。采用二甲基噻唑-二苯基溴化四氮唑（MTT）法测定人成骨肉瘤MG63细胞株的细胞活力。结果：阳极氧化后形成无定形纳米结构的氧化钛，热处理后转变为锐钛矿和金红石相。在SBF中浸泡后，阳极化样品表面形成球形无定形磷酸钙聚集体，在阳极化退火样品表面形成结晶羟基磷灰石。样品未检测到细胞毒性。结论：阳极氧化后热处理可作为一种有效的表面处理工艺，提高钛颗粒骨组织修复和增强的生物活性。
Purpose: The highly porous titanium granules are currently being used as bone substitute material and for bone tissue augmentation. However, they suffer from weak bone bonding ability. The aim of this study was to create a nanostructured surface oxide layer on irregularly shaped titanium granules to improve their bioactivity. This could be achieved using optimized electrochemical anodic oxidation (anodizing) and heat treatment processes. Materials and Methods: The anodizing process was done in an ethylene glycol-based electrolyte at an optimized condition of 60 V for 3 hours. The anodized granules were subsequently annealed at 450 degrees C for 1 hour. Scanning electron microscopy (SEM), energy-dispersive x-ray spectroscopy (EDS), and x-ray diffraction (XRD) were used to characterize the surface structure and morphology of the granules. The in vitro bioactivity of the samples was evaluated by immersion of specimens in simulated body fluid (SBF) for 1, 2, and 3 weeks. The human osteoblastic sarcoma cell line, MG63, was used to evaluate cell viability on the samples using dimethylthiazol-diphenyl tetrazolium bromide (MTT) assay. Results: The results demonstrated the formation of amorphous nanostructured titanium oxide after anodizing, which transformed to crystalline anatase and rutile phases upon heat treatment. After immersion in SBF, spherical aggregates of amorphous calcium phosphate were formed on the surface of the anodized sample, which turned into crystalline hydroxyapatite on the surface of the anodized annealed sample. No cytotoxicity was detected among the samples. Conclusion: It is suggested that anodic oxidation followed by heat treatment could be used as an effective surface treatment procedure to improve bioactivity of titanium granules implemented for bone tissue repair and augmentation.