Expression of follicle-stimulating hormone receptor in human ovary
Expression of follicle-stimulating hormone receptor in human ovary
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DOI：
10.1046/j.1365-2362.1997.1350682.x
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发表时间：
1997-06-01
影响因子：
5.5
通讯作者：
Miyamoto, K
中科院分区：
文献类型：
--
作者：
Minegishi, T;Tano, M;Miyamoto, K
促性腺激素，即卵泡刺激素(FSH)和黄体生成素(LH)，是调节卵巢功能的关键激素。卵泡发生过程中FSH受体的获得被认为是卵泡后续发育的关键事件。然而，由于人类FSH受体的丰度低以及人体组织的可获得性有限，这些受体的结合和生化特性尚未得到很好的表征。结合实验表明，尽管FSH受体的亲和力在月经周期中没有变化，但卵泡中期的FSH受体总数比其他时期增加了约一倍。Northern印迹分析检测FSH受体mRNA的相对水平，原位杂交定位FSH受体转录本。对人卵巢的Northern杂交分析检测到FSH受体的两个转录本(4.1和2.4kb)。FSH受体mRNA在排卵前卵泡中含量丰富，在黄体中的表达降低约50%。原位杂交发现，FSH受体mRNA定位于发育中卵泡的颗粒细胞。采用逆转录-聚合酶链式反应技术检测不同亚型的FSH受体在人黄体和胎盘中的表达。
The gonadotropins, follicle-stimulating hormone (FSH) and luteinizing hormone (LH), are key hormones in the regulation of ovarian function. The acquisition of FSH receptors during folliculogenesis is believed to be a key event in the subsequent development of the follicle. However, the binding and biochemical properties of the human FSH receptor are not well-characterized owing to the low abundance of these receptors and the Limited availability of human tissue. The binding experiments show that, while the affinity of the FSH receptor does not change through the menstrual cycle, the total number of FSH receptors in the leading follicles increases by about two-fold at mid-follicular phase compared with those at other periods. Northern blot analysis was used to measure relative levels of FSH receptor mRNA, and in situ hybridization was used to localize FSH receptor transcripts. Northern blot analysis of human ovaries detected two transcripts (4.1 and 2.4 kb) for the FSH receptor. The FSH receptor mRNA was abundant in the preovulatory follicle, with expression decreased by about 50% in the corpus luteum. Using in situ hybridization, FSH receptor mRNA was found to be confined to the granulosa cells of developing follicles. A reverse transcription-polymerase chain reaction amplification was used to detect the expression of different isoforms of the FSH receptor mRNA in human corpus luteum and placenta.