Collagen sponge scaffolds containing growth factors for the functional regeneration of tracheal epithelium.
Collagen sponge scaffolds containing growth factors for the functional regeneration of tracheal epithelium.
复制标题
含有生长因子的胶原海绵支架，用于气管上皮的功能再生。
DOI：
10.1002/term.2835
复制
发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Omori K.
中科院分区：
文献类型：
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作者：
Nakamura R;Katsuno T;Kitamura M;Yamashita M;Tsuji T;Suzuki R;Kishimoto Y;Suehiro A;Tateya I;Nakamura T;Omori K.
气管上皮细胞具有屏障和粘膜纤毛清除功能，可防止外来颗粒和感染性物质的侵入。因此，在气管重建后，需要上皮的功能和形态再生来防止呼吸下降和感染性疾病。虽然生长因子（GF）促进气管上皮形态的再生，但含有GF的气管移植物是否有利于气管上皮功能的再生仍不清楚。因此，我们制造了含有胰岛素样GF-1（IGF-1）和碱性成纤维细胞、肝细胞和表皮GF（分别为bFGF、HGF和EGF）的胶原海绵支架，并评估了移植物对气管上皮功能性再生的影响。手术造成部分气管缺损，然后将含有IGF-1、bFGF、HGF或EGF的胶原海绵移植到缺损部位。随后的免疫荧光研究表明，表皮生长因子和碱性成纤维细胞生长因子有助于紧密连接分子的规则分布，和示踪渗透性试验表明，表皮生长因子和碱性成纤维细胞生长因子促进再生的屏障功能。在用EGF和bFGF补充的胶原海绵处理的重建区域中，使用扫描电子显微镜也观察到纤毛发生增加。然而，补充碱性成纤维细胞生长因子的胶原海绵比补充表皮生长因子的海绵产生更大的微球运输。提示含bFGF的胶原海绵支架可促进气管上皮组织的功能性再生。
Tracheal epithelia have barrier and mucociliary clearance functions that prevent invasion of extraneous particles and infectious materials. Hence, following tracheal reconstructions, functional and morphological regeneration of epithelia is required to prevent respiratory declines and infectious diseases. Although growth factors (GFs) promote the regeneration of tracheal epithelial morphologies, it remains unclear whether tracheal grafts containing GFs are beneficial for regeneration of tracheal epithelial functions. Thus, we fabricated collagen sponge scaffolds containing insulin‐like GF‐1 (IGF‐1) and the basic fibroblast, hepatocyte, and epidermal GFs (bFGFs, HGFs, and EGFs, respectively), and we evaluated the effects of the grafts on the functional regeneration of tracheal epithelia. Partial tracheal defects were imposed surgically, and collagen sponges containing IGF‐1, bFGF, HGF, or EGF were then transplanted to defect sites. Subsequent immunofluorescence studies suggested that EGF and bFGF contribute to regular distributions of tight junction molecules, and tracer permeability assays suggested that EGF and bFGF promote regeneration of barrier function. Increased ciliogenesis was also observed using scanning electron microscopy in reconstructed regions treated with EGF‐ and bFGF‐supplemented collagen sponges. However, bFGF‐supplemented collagen sponges led to greater microsphere transport than did EGF‐supplemented sponges. The present data suggested that collagen sponge scaffold containing bFGF promotes functional regeneration of tracheal epithelial tissues.