The high molecular mass rhoptry protein, RhopH1, is encoded by members of the clag multigene family in Plasmodium falciparum and Plasmodium yoelii
The high molecular mass rhoptry protein, RhopH1, is encoded by members of the clag multigene family in Plasmodium falciparum and Plasmodium yoelii
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DOI：
10.1016/s0166-6851(01)00391-7
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发表时间：
2001-12-01
影响因子：
1.5
通讯作者：
Torii, M
中科院分区：
文献类型：
--
作者：
Kaneko, O;Tsuboi, T;Torii, M
疟疾裂殖子棒状体含有一种高分子量的蛋白质复合物，称为RhopH。RhopH由三种多肽RhopH 1、RhopH 2组成。和RhopH 3，由不同的基因编码。使用单克隆抗体纯化的蛋白质复合物从恶性疟原虫和约氏疟原虫，肽通过消化RhopH 1和它们的序列确定通过质谱或Edman降解。在这两个物种的基因编码RhopH 1被确定为细胞粘附连锁无性基因（clag）家族的成员。在恶性疟原虫中，3号染色体上的家族成员被鉴定为编码RhopH 1。在约氏疟原虫中鉴定了两个相关基因并测序。其中一个基因pyrhoph 1a通过肽分析被阳性鉴定为编码RhopH 1，另一个基因pyrhoph 1a-p至少被转录。存在于两种寄生虫物种中的clag家族中的基因具有许多保守特征。证实了棒状体中约氏疟原虫蛋白复合物的大小和位置。在9号染色体上发现的第一个clag基因涉及细胞粘附，感染的红细胞与宿主内皮细胞的结合;这项研究表明，该家族的其他成员编码裂殖子棒状体蛋白，这些蛋白可能参与裂殖子-红细胞相互作用。我们建议将该家族重新命名为rhoph 1/clag。(C)2001 Elsevier Science B. V.保留所有权利。
Malarial merozoite rhoptries contain a high molecular mass protein complex called RhopH. RhopH is composed of three polypeptides, RhopH1, RhopH2. and RhopH3, encoded by distinct genes. Using monoclonal antibody-purified protein complex from both Plasmodium falciparum and Plasmodium yoelii, peptides were obtained by digestion of RhopH1 and their sequence determined either by mass spectrometry or Edman degradation. In both species the genes encoding RhopH1 were identified as members of the cytoadherence linked asexual gene (clag) family. In P. falciparum the family members on chromosome 3 were identified as encoding RhopH1. In P. yoelii two related genes were identified and sequenced. One of the genes, pyrhoph 1a, was positively identified as encoding RhopH1 by the peptide analysis and the other gene, pyrhoph 1a-p, was at least transcribed. Genes in the clag family present in both parasite species have a number of conserved features. The size and location of the P. yoelii protein complex in the rhoptries was confirmed. The first clag gene identified on chromosome 9 was implicated in cytoadherence, the binding of infected erythrocytes to host endothelial cells; this study shows that other members of the family encode merozoite rhoptry proteins, proteins that may be involved in merozoite-erythrocyte interactions. We propose that the family should be renamed as rhoph1/clag. (C) 2001 Elsevier Science B.V. All rights reserved.