Silver nanoparticles, a promising treatment against clinically important fluconazole-resistant Candida glabrata
Silver nanoparticles, a promising treatment against clinically important fluconazole-resistant Candida glabrata
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DOI：
10.1111/lam.13560
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发表时间：
2021-09-18
影响因子：
2.4
通讯作者：
Alkilany, A. M.
中科院分区：
文献类型：
--
作者：
Darwish, R. M.;AlKawareek, M. Y.;Alkilany, A. M.
对唑类抗真菌药物的耐药性是光滑念珠菌治疗的一个挑战性限制。它与细胞质膜上外排泵过度表达导致的抗真菌剂细胞内浓度降低有关。这项工作评估了银纳米颗粒（AgNPs）逆转真菌细胞对氟康唑耐药性的潜力。采用湿化学法制备了银纳米粒子，并用紫外可见分光光度法、动态光散射法和zeta电位法对其进行了表征。采用微量肉汤稀释法和倾注平板法研究了两种C.已知过表达主动外排泵的glabrata氟康唑抗性菌株（DSY 565和CBS 138）和标准氟康唑敏感菌株ATCC 22553。银纳米颗粒-氟康唑组合显著降低了氟康唑的浓度而不损害活性。这些结果表明，银纳米颗粒增强氟康唑的疗效，并提供了一个有前途的应用在治疗C。glabrata感染
Resistance to azole antifungal agents is a challenging limitation in Candida glabrata treatment. It is associated with decreased intracellular concentrations of antifungal agents as a result of overexpression of efflux pumps on the cellular plasma membranes. This work evaluates the potential of silver nanoparticles (AgNPs) to reverse the resistance of fungal cells to fluconazole. Silver nanoparticles were prepared using wet chemical method and characterised by UV-Vis spectrophotometry, dynamic light scattering, and zeta potential. Broth microdilution and pour plates methods were used to study the anticandidal activity using two C. glabrata fluconazole-resistant strains (DSY565 and CBS138) known to overexpress active efflux pumps, and a standard fluconazole sensitive strain ATCC 22553. Silver nanoparticles-fluconazole combinations decreased concentrations of fluconazole substantially without compromising the activity. These findings suggest that AgNPs enhance the efficacy of fluconazole and offer a promising application in therapy of C. glabrata infections.