In vivo assessment of absolute perfusion and intracapillary blood volume in the murine myocardium by spin labeling magnetic resonance imaging
In vivo assessment of absolute perfusion and intracapillary blood volume in the murine myocardium by spin labeling magnetic resonance imaging
复制标题
DOI：
10.1002/mrm.20327
复制
发表时间：
2005-03-01
影响因子：
3.3
通讯作者：
Bauer, WR
中科院分区：
文献类型：
--
作者：
Streif, JUG;Nahrendorf, M;Bauer, WR
采用自旋标记磁共振成像技术，测定了小鼠在体心肌的绝对灌注和毛细血管内或局部血容量（RBV）。基于像素的灌注和RBV地图计算像素分辨率为469 × 469妈妈和切片厚度为2毫米。T，成像模块是一个分段的反转恢复快照快速低角度拍摄序列与速度补偿在所有三个梯度方向。基线时的组平均心肌灌注测定为701 +/- 53 mL（100 g . min）（-1），用于异氟烷麻醉（N = 11），平均心率（HR）为455 +/- 10次/分钟（bpm）。该值与通过侵入性微球检查确定的灌注值非常一致。对于静脉注射麻醉剂丙泊酚，基线灌注降至383 +/- 40 mL（100 g . min）（-1）（N = 17，P < 0.05 vs.异氟醚），平均心率为261 +/-13 bpm（P < 0.05，相对于异氟醚）。此外，6只心肌梗死小鼠在异氟烷麻醉（HR 397 7 bpm）下进行了研究。灌注图显示梗死区灌注明显减少。远端心肌的灌注显著降低至476 +/- 81 mL（100 g . min）（-1）（P < 0.05 vs. sham）。关于局部血容量，丙泊酚麻醉下健康小鼠心肌的平均值为11.8 ± 0.8 vol %（N = 4，HR 233 ± 17 bpm）。总的来说，所提出的技术提供了非侵入性的在体内评估的灌注和区域血容量在小鼠心肌的第一次，似乎是有前途的工具，在心血管研究中的小鼠模型的表征。
The absolute perfusion and the intracapillary or regional blood volume (RBV) in murine myocardium were assessed in vivo by spin labeling magnetic resonance imaging. Pixel-based perfusion and RBV maps were calculated at a pixel resolution of 469 x 469 mum and a slice thickness of 2 mm. The T, imaging module was a segmented inversion recovery snapshot fast low angle shot sequence with velocity compensation in all three gradient directions. The group average myocardial perfusion at baseline was determined to be 701 +/- 53 mL (100 g . min)(-1) for anesthesia with isoflurane (N = 11) at a mean heart rate (HR) of 455 +/- 10 beats per minute (bpm). This value is in good agreement with perfusion values determined by invasive microspheres examinations. For i.v. administration of the anesthetic Propofol, the baseline perfusion decreased to 383 +/- 40 mL (100 g . min)(-1) (N = 17, P < 0.05 versus. isoflurane) at a mean heart rate of 261 +/- 13 bpm (P < 0.05 versus isoflurane). In addition, six mice with myocardial infarction were studied under isoflurane anesthesia (HR 397 7 bpm). The perfusion maps showed a clear decrease of the perfusion in the infarcted area. The perfusion in the remote myocardium decreased significantly to 476 +/- 81 mL (100 g . min)(-1) (P < 0.05 versus sham). Regarding the regional blood volume, a mean value of 11.8 0.8 vol % was determined for healthy murine myocardium under anesthesia with Propofol (N = 4, HR 233 17 bpm). In total, the presented techniques provide noninvasive in vivo assessment of the perfusion and the regional blood volume in the murine myocardium for the first time and seem to be promising tools for the characterization of mouse models in cardiovascular research.