Genetic ablation of flowers in transgenic Arabidopsis
Genetic ablation of flowers in transgenic Arabidopsis
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DOI：
10.1046/j.1365-313x.1998.00260.x
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发表时间：
1998-09-01
期刊：
影响因子：
7.2
通讯作者：
Weigel, D
中科院分区：
文献类型：
--
作者：
Nilsson, O;Wu, E;Weigel, D
我们已经创建了转基因拟南芥植物，其中编码细胞自主白喉毒素 A 链 (DT-A) 的基因在 LEAFY (LFY) 启动子的控制下表达。该启动子在新出现的叶原基和幼花中均具有活性，其中在花中活性最高。大多数 LFY:DT-A 植物具有正常的营养发育，但缺乏花，这表明启动子相对广泛的活性并不排除其用于消融选定组织的可能性，只要不同组织之间的活性水平差异显着。我们还发现 LFY:DT-A 植物中的花被空苞片取代，这表明花源信号通常会抑制拟南芥中的苞片发育。
We have created transgenic Arabidopsis plants in which a gene encoding the cell-autonomous diphtheria toxin A chain (DT-A) was expressed under the control of the LEAFY (LFY) promoter. This promoter is active both in emerging leaf primordia and young flowers, with the highest activity in flowers. The majority of LFY:DT-A plants had normal vegetative development but lacked flowers, demonstrating that relatively widespread activity of a promoter does not exclude its possible use for ablating selected tissues, as long as differences in activity levels between different tissues are significant. We also found that flowers were replaced by empty bracts in LFY:DT-A plants, suggesting that flower-derived signals normally suppress bract development in Arabidopsis.