Xenopus TFIIIA gene transcription is dependent on cis-element positioning and chromatin structure.
Xenopus TFIIIA gene transcription is dependent on cis-element positioning and chromatin structure.
复制标题
非洲爪蟾 TFIIIA 基因转录依赖于顺式元件定位和染色质结构。
DOI：
10.1128/mcb.18.7.3811
复制
发表时间：
1998
影响因子：
5.3
通讯作者：
Taylor,WL
中科院分区：
文献类型：
--
作者：
Pfaff,SL;Taylor,WL
TheXenopusTFIIIA gene is transcribed very efficiently in oocytes. In addition to a TATA element at −30, we show that from −425 to +7 the TFIIIA gene contains only two positiveciselements centered at −267 (element 1) and −230 (element 2). This arrangement of theciselements in the TFIIIA gene is striking because these two elements are positioned very close to each other yet separated from the TATA element by approximately 190 nucleotides. We show that the 190-nucleotide spacing between the TATA element and the upstreamciselements (elements 1 and 2) is critical for efficient transcription of the gene in oocytes and that a nucleosome is positioned in this intervening region. This nucleosome may act positively on TFIIIA transcription in oocytes by placing transcription factors bound at elements 1 and 2 in a favorable position relative to the transcription complex at the TATA element.
