Detection of size spectrum of microalgae cells in an integrated underwater microfluidic device
Detection of size spectrum of microalgae cells in an integrated underwater microfluidic device
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集成水下微流体装置中微藻细胞尺寸谱的检测
DOI：
10.1016/j.jembe.2015.08.016
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发表时间：
2015-12-01
影响因子：
2
通讯作者：
Li, Dongqing
中科院分区：
文献类型：
--
作者：
Wang, Junsheng;Song, Younan;Li, Dongqing
生物颗粒的粒径谱直接影响海洋生态系统的营养循环和能流分布。快速准确地检测海洋浮游植物的大小和分布，对于深入研究海洋浮游植物的生理特征、群落结构和功能具有非常重要的作用。提出了一种基于微流控芯片的微藻细胞尺寸谱检测方法和系统。该装置采用微流控电阻脉冲传感器（RPS）技术测定微藻细胞粒径，通过叶绿素荧光强度判断颗粒是否为活微藻细胞。该系统主要包括微流控芯片平台、样品采集模块、光电检测单元、数据采集、数据处理和系统控制模块。为了证明所开发的系统的性能，实验进行了与三种微藻细胞（等鞭金藻，杜氏藻桑拿和四藻chui），典型的浮游植物细胞在海洋环境中，作为样品。并与商用流式细胞仪进行了对比实验。实验结果表明，该系统可靠、准确。目前的装置对微藻细胞大小的检测限为3 μ m，未来可以进一步提高。该微流控系统具有检测速度快、体积小、操作简便等优点，为微藻细胞尺寸谱检测提供了一种非常有前途的方法。在进一步产品开发之前，它具有很大的潜力，用于水下原位检测微型植物浮游生物。(C)2015 Elsevier B.V.版权所有。
Size spectrum of biological particles directly affects nutrient cycling and energy flow distribution of marine ecosystems. Fast and accurate detection of the size and distribution of marine phytoplankton plays a very important role for in-depth study of physiological characteristics of marine phytoplankton community structure and function. In this paper, a novel detection method and system of size spectrum of microalgae cells on a microfluidic chip is presented. In this device, a microfluidic resistance pulse sensor (RPS) technology is adopted to determine the particle size of microalgae cells; chlorophyll fluorescence intensity is used to judge if a particle is a live microalgae cell or not. Inside a waterproof case, the system mainly comprises a microfluidic chip platform, a sample acquisition module, an optoelectronic detection unit, and a module performing data acquisition, data processing and system control. To demonstrate the performance of the developed system, experiments were conducted with three species of microalgae cells (Isochrysis galbana, Dunaliella sauna and Tetraselmis chui), typical phytoplankton cells in marine environments, as samples. Comparison experiments between the developed detection system and a commercial flow cytometer were conducted. The results demonstrate that the developed system is reliable and accurate. The limit of detection of microalgae cell size is 3 tun for the current device and can be improved in the future. The presented microfluidic system provides a very promising method of size spectrum detection of microalgae cells with the advantages such as fast detection, small size and easy operation. It has a great potential for in-situ underwater detection of micro phytoplanktons pending further product development. (C) 2015 Elsevier B.V. All rights reserved.