Evolutionary and Experimental Loss of Gene Body Methylation and Its Consequence to Gene Expression
Evolutionary and Experimental Loss of Gene Body Methylation and Its Consequence to Gene Expression
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DOI：
10.1534/g3.119.400365
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发表时间：
2019-08-01
影响因子：
2.6
通讯作者：
Schmitz, Robert J.
中科院分区：
文献类型：
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作者：
Bewick, Adam J.;Zhang, Yinwen;Schmitz, Robert J.
在开花植物中，基因体甲基化（gbM）与组成型表达基因的子集相关。已经提出gbM调节基因表达。在这里，我们表明，有没有一致的和直接的差异表达以下的损失gbM。通过比较gbM基因在拟南芥加入orthopathic基因在两个盐地黄草基因型的表达，我们确定了积极和消极的表达差异与gbM损失。然而，表达在很大程度上不受E.盐柳碱物种间的表达差异在A.显示gbM变异的拟南芥种质。此外，与野生型相比，实验诱导的gbM损失并不一致地导致表达差异。迄今为止，没有令人信服的数据来支持gbM的存在/不存在和开花植物中的表达调节之间的直接因果关系。
In flowering plants, gene body methylation (gbM) is associated with a subset of constitutively expressed genes. It has been proposed that gbM modulates gene expression. Here, we show that there are no consistent and direct differences to expression following the loss of gbM. By comparing expression of gbM genes in Arabidopsis thaliana accessions to orthologous genes in two Eutrema salsugineum genotypes, we identified both positive and negative expression differences associated with gbM loss. However, expression is largely unaffected by gbM loss in E. salsugineum. Expression differences between species were within the variation of expression observed within A. thaliana accessions that displayed variation in gbM. Furthermore, experimentally induced loss of gbM did not consistently lead to differences in expression compared to wild type. To date, there is no convincing data to support a direct causal link between the presence/absence of gbM and the modulation of expression in flowering plants.