Production of sophorolipids from non-edible jatropha oil by Stamerella bombicola NBRC 10243 and evaluation of their interfacial properties.
Production of sophorolipids from non-edible jatropha oil by Stamerella bombicola NBRC 10243 and evaluation of their interfacial properties.
复制标题
利用 Stamerella bombicola NBRC 10243 从非食用麻风树油中生产槐糖脂并评估其界面特性。
DOI：
10.5650/jos.62.857
复制
发表时间：
2013
影响因子：
1.5
通讯作者：
D. Kitamoto
中科院分区：
文献类型：
--
作者：
T. Imura;D. Kawamura;Tomotake Morita;Shun Sato;T. Fukuoka;Yosuke Yamagata;Makoto Takahashi;Koji Wada;D. Kitamoto
为了促进从可再生和廉价的自然资源中开发生物基化学品，我们寻求使用不可食用的麻疯树油生产生物表面活性剂。本研究对已知的20种产生生物表面活性剂的酵母进行了测试，发现bombicola Stamerella NBRC 10243可以有效地利用麻风树油生成内酯型SL （L-SL）和酸型SL （a -SL）的混合物sophorolids （SL）。在以米糠为有机营养源的培养条件下，5-L罐式发酵罐培养9 d后，SLs产量达到122.6 g/L。高效液相色谱分析表明，随着培养时间的延长，油中主要有毒成分磷酸酯（phorbol esters, PEs）的含量显著降低，表明酵母对磷酸酯有降解作用。虽然溶剂萃取培养基得到的SLs中含有少量PE，但经碱性处理（5N NaOH, 80°C）得到的a - sl （a - sl - na）钠盐在HPLC分析中没有PE峰。A-SL-Na具有优良的表面活性，具有低CMC （9.0×10⁻⁴M）和γ(CMC) (29.6 mN/ M)，低于十二烷基硫酸钠（SDS）。A-SL-Na对辛酸（[辛酸]/[A-SL-Na]）的增溶能力为2.0，是SDS的一半。我们的发现将有助于提高从非食用原料中生产SL，并扩大有前途的生物基表面活性剂的使用。
To facilitate the development of bio-based chemicals from renewable and inexpensive natural resources, we sought to produce biosurfactants using non-edible jatropha oil. Twenty yeasts known to produce biosurfactants were tested in this study, and Stamerella bombicola NBRC 10243 was found to use jatropha oil efficiently to produce sophorolipids (SLs) as a mixture of lactone-form SL (L-SL) and acid-form SL (A-SL). Under culture conditions using rice bran as the source of organic nutrients, the yield of SLs reached 122.6 g/L in 5-L jar fermentors after 9 d in culture. HPLC analysis of the culture medium showed that the levels of phorbol esters (PEs), major toxic components of the oil, decreased markedly with an increase in culture time, suggesting that the yeast degrades PEs. Although the SLs obtained by solvent extraction of the culture medium contained a small amount of PEs, the sodium salt of A-SL (A-SL-Na) obtained by alkaline treatment (5N NaOH, 80°C) showed no PE peaks upon HPLC analysis. A-SL-Na had excellent surface activity with low CMC (9.0×10⁻⁴ M) and γ(CMC) (29.6 mN/m), which are lower than that of sodium dodecyl sulfate (SDS). The solubilizing ability of A-SL-Na toward for octanoic acid ([octanoic acid]/[A-SL-Na]) was found to be 2.0, which is half that of SDS. Our findings should help improve SL production from non-edible feedstock and broaden the use of promising bio-based surfactants.