Horseradish peroxidase: a modern view of a classic enzyme
Horseradish peroxidase: a modern view of a classic enzyme
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DOI：
10.1016/j.phytochem.2003.10.022
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发表时间：
2004-02-01
期刊：
影响因子：
3.8
通讯作者：
Veitch, NC
中科院分区：
文献类型：
--
作者：
Veitch, NC
辣根过氧化物酶是一种重要的含血红素酶，已被研究了世纪。近年来，在酶及其催化中间体的三维结构、催化机制和特定氨基酸残基的功能方面有了新的信息。目前，定点突变和定向进化技术已被广泛应用于辣根过氧化物酶的结构和功能研究，并为开发工程酶在天然产物和精细化学品合成、医学诊断和生物修复中的实际应用提供了机会。目前正在评估辣根过氧化物酶和吲哚-3-乙酸或其衍生物的组合作为用于靶向癌症治疗的药剂。传统上与酶相关的生理作用，包括吲哚-3-乙酸代谢，生物聚合物的交联和木质化，在分子水平上得到更好的理解，但在这些过程中的特定辣根过氧化物酶同工酶的参与尚未明确界定。在这方面的进展应导致整个过氧化物酶基因家族的拟南芥，现已完成的鉴定。(C)2003 Elsevier Ltd.保留所有权利。
Horseradish peroxidase is an important heme-containing enzyme that has been studied for more than a century. In recent years new information has become available on the three-dimensional structure of the enzyme and its catalytic intermediates, mechanisms of catalysis and the function of specific amino acid residues. Site-directed mutagenesis and directed evolution techniques are now used routinely to investigate the structure and function of horseradish peroxidase and offer the opportunity to develop engineered enzymes for practical applications in natural product and fine chemicals synthesis, medical diagnostics and bioremediation. A combination of horseradish peroxidase and indole-3-acetic acid or its derivatives is currently being evaluated as an agent for use in targeted cancer therapies. Physiological roles traditionally associated with the enzyme that include indole-3-acetic acid metabolism, cross-linking of biological polymers and lignification are becoming better understood at the molecular level, but the involvement of specific horseradish peroxidase isoenzymes in these processes is not yet clearly defined. Progress in this area should result from the identification of the entire peroxidase gene family of Arabidopsis thaliana, which has now been completed. (C) 2003 Elsevier Ltd. All rights reserved.