Immunohistochemical demonstration of ranavirus antigen in the tissues of infected frogs (Rana temporaria) with systemic haemorrhagic or cutaneous ulcerative disease
Immunohistochemical demonstration of ranavirus antigen in the tissues of infected frogs (Rana temporaria) with systemic haemorrhagic or cutaneous ulcerative disease
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DOI：
10.1016/j.jcpa.2007.08.003
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发表时间：
2008-01-01
影响因子：
0.8
通讯作者：
Russell, P. H.
中科院分区：
文献类型：
--
作者：
Cunningham, A. A.;Tems, C. A.;Russell, P. H.
20世纪80年代末，蛙病毒病成为英国常见青蛙（蛙属）每年重复流行性死亡的原因。受影响的青蛙目前与急性疾病的特点是全身出血，或与慢性疾病的特点是皮肤溃疡，但没有内部的肉眼病变。普通蟾蜍（Bufo bufo）也被发现患有出血性蛙病毒病。为了研究可能的差异，在发病机制的蛙病毒感染的每一个主要疾病综合征，我们研究了一系列的组织，从自然和实验感染的青蛙使用抗蛙病毒免疫组化。蛙病毒位于各种细胞，包括纤维细胞，上皮细胞，淋巴细胞，肝细胞和黑素-巨噬细胞，但较少的组织中感染的青蛙皮肤溃疡综合征比青蛙全身出血性疾病。具体而言，与出血综合征的青蛙相反，溃疡综合征的青蛙的脾淋巴细胞、胰腺或胃肠道上皮细胞中没有病毒抗原的标记。在患有系统性出血性疾病的青蛙的肝、肾、胰腺和胃中观察到胞浆内病毒包涵体，但在患有溃疡综合征的青蛙中没有。免疫组织化学标记选定的组织从受影响的蟾蜍表现出Ranavirus抗原的皮肤和内脏。该技术表明，与蛙病毒溃疡综合征相比，出血形式的蛙病毒病与更广泛的内脏器官的病毒感染有关，并确定了某些组织（如脾脏）的感染，这可能在出血性疾病的发病机制中很重要。(C)2007爱思唯尔有限公司版权所有。
Ranavirus disease emerged as a cause of annually recurring epidemic mortality of common frogs (Rana temporaria) in Britain in the late-1980s. Affected frogs present with a peracute disease characterized by systemic haemorrhage, or with a chronic disease characterized by skin ulceration, but no internal gross lesions. Common toads (Bufo bufo) have also been found with haemorrhagic ranavirus disease. In order to investigate possible differences in the pathogenesis of ranavirus infection for each main disease syndrome, we studied a range of tissues from both naturally and experimentally infected frogs using anti-ranavirus immunohistochemistry. Ranavirus was located in a variety of cells, including fibrocytes, epithelial cells, lymphocytes, hepatocytes and melano-macrophages, but fewer tissues were infected in frogs with the skin ulcerative syndrome than in frogs with systemic haemorrhagic disease. Specifically, and in contrast to frogs with haemorrhagic syndrome, there was no labelling for viral antigen in the splenic lymphocytes, pancreas or gastrointestinal epithelium in frogs with ulcerative syndrome. Intracytoplasmic virus inclusions were seen in the liver, kidney, pancreas and stomach of frogs with systemic haemorrhagic disease, but not in frogs with the ulcerative syndrome. Immunohistochemical labelling of selected tissues from an affected toad demonstrated ranavirus antigen in the skin and viscera. This technique demonstrates that, in comparison to ranavirus ulcerative syndrome, the haemorrhagic form of ranavirus disease is associated with virus infection of a wider range of internal organs and identifies the infection of certain tissues, such as the spleen, which might be important in the pathogenesis of the haemorrhagic disease. (C) 2007 Elsevier Ltd. All rights reserved.