Novel apatite fiber scaffolds can promote three-dimensional proliferation of osteoblasts in rodent bone regeneration models.
Novel apatite fiber scaffolds can promote three-dimensional proliferation of osteoblasts in rodent bone regeneration models.
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DOI：
10.1002/jbm.a.32147
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发表时间：
2009-09
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影响因子：
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通讯作者：
H. Morisue;M. Matsumoto;K. Chiba;H. Matsumoto;Y. Toyama;M. Aizawa;N. Kanzawa;Takahiro J. Fujimi;H. Uchida;I. Okada
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文献类型：
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作者：
H. Morisue;M. Matsumoto;K. Chiba;H. Matsumoto;Y. Toyama;M. Aizawa;N. Kanzawa;Takahiro J. Fujimi;H. Uchida;I. Okada
采用均匀沉淀法成功地合成了羟基磷灰石纤维。使用这些羟基磷灰石纤维，我们已经生产了磷灰石纤维支架（AFS）具有良好的控制孔径（孔隙率超过95%）。AFS的合成相对简单，其孔隙率和孔径是可控的。通过（1）在体外将细胞接种于AFS中并培养，（2）将接种有细胞的AFS植入小鼠皮下组织中，评价AFS作为骨再生支架的有用性。AFS具有支持细胞粘附、增殖和分化的生物相容性。植入皮下12周后，AFS内可形成异位成骨。由于其高的孔间连接、孔径和孔隙率，认为AFS是提供三维细胞培养环境的有效支架。在体外和体内环境中，多孔AFS更有利于细胞增殖，细胞粘附，增殖能力，细胞分化，最终诱导骨长入支架内。
We have successfully synthesized hydroxyapatite fibers via a homogenous precipitation method. Using these hydroxyapatite fibers, we have produced the apatite fiber scaffolds (AFS) with well-controlled pore sizes (porosity above 95%). The AFS is relatively simple to synthesize, and its porosity and pore size are controllable. The usefulness of AFS as a scaffold for bone regeneration was evaluated by (1) seeding and culturing cells in the AFS in vitro, (2) implanting the AFS seeded with cells inside the subcutaneous tissue of mice. The AFS had biocompatibility to support cell adhesion, proliferation, and differentiation. Ectopic bone formation could be formed in the AFS at 12 weeks after implantation into the subcutaneous tissue. Because of its high interpore connection, pore diameters, and porosity, it was believed that AFS was an effective scaffold that provided a three-dimensional cell culture environment. In both in vitro and in vivo environments, the more porous AFS was more advantageous in cell proliferation, cell adhesion, proliferating capacity, robust cell differentiation, ultimately inducing bone ingrowth inside the scaffolds.