Near-Infrared Light-Activated Thermosensitive Liposomes as Efficient Agents for Photothermal and Antibiotic Synergistic Therapy of Bacterial Biofilm
Near-Infrared Light-Activated Thermosensitive Liposomes as Efficient Agents for Photothermal and Antibiotic Synergistic Therapy of Bacterial Biofilm
复制标题
近红外光激活热敏脂质体作为细菌生物膜光热和抗生素协同治疗的有效药物
DOI：
10.1021/acsami.8b01327
复制
发表时间：
2018
影响因子：
9.5
通讯作者：
Li Chaoxing
中科院分区：
文献类型：
--
作者：
Zhao Yu;Dai Xiaomei;Wei Xiaosong;Yu Yunjian;Chen Xuelei;Zhang Xinge;Li Chaoxing
生物膜与慢性感染密切相关，难以根除。开发有效的治疗策略来控制生物膜感染仍然具有挑战性。针对细菌生物膜结构，设计并制备了具有光热和抗生素协同治疗能力的近红外激活热敏脂质体，以消除铜绿假单胞菌生物膜。带正电荷且体积小的脂质体有助于进入生物膜微通道，在感染部位局部释放抗生素。脂质体在37 °C下保持稳定，在45 °C以上释放约80%的抗生素。生物膜分散率高达80%，与单独过量抗生素相比增加了7至8倍，表明局部抗生素释放和光热共治疗提高了抗菌效率。体内载药脂质体治疗P.结果显示，对肺脓肿有显著的治疗效果。此外，光热处理可刺激bcl 2相关的athanogene 3的表达，以防止正常组织的热损伤。近红外激活纳米粒子载体具有巨大的治疗潜力，可以通过热触发药物释放和光热治疗显著提高抗生素的疗效。
Biofilm is closely related to chronic infections and is difficult to eradicate. Development of effective therapy strategies to control biofilm infection is still challenging. Aiming at biofilm architecture, we designed and prepared near-infrared-activated thermosensitive liposomes with photothermal and antibiotic synergistic therapy capacity to eliminatePseudomonas aeruginosabiofilm. The liposomes with positive charge and small size aided to enter the biofilm microchannels and locally released antibiotics in infection site. The liposomes could remain stable at 37 °C and release about 80% antibiotics over 45 °C. The biofilm dispersion rate was up to 80%, which was a 7- to 8-fold rise compared to excess antibiotic alone, indicating that the localized antibiotic release and photothermal co-therapy improved the antimicrobial efficiency. In vivo drug-loaded liposomes in treatingP. aeruginosa-induced abscess exhibited an outstanding therapeutic effect. Furthermore, photothermal treatment could stimulate the expression of bcl2-associated athanogene 3 to prevent normal tissue from thermal damage. The near-infrared-activated nanoparticle carriers had the tremendous therapeutic potential to dramatically enhance the efficacy of antibiotics through thermos-triggered drug release and photothermal therapy.