A family of human β3-galactosyltransferases -: Characterization of four members of a UDP-galactose:β-N-acetyl-glucosamine/βN-acetyl-galactosamine β-1,3-galactosyltransferase family
A family of human β3-galactosyltransferases -: Characterization of four members of a UDP-galactose:β-N-acetyl-glucosamine/βN-acetyl-galactosamine β-1,3-galactosyltransferase family
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DOI：
10.1074/jbc.273.21.12770
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发表时间：
1998-05-22
影响因子：
4.8
通讯作者：
Clausen, H
中科院分区：
文献类型：
--
作者：
Amado, M;Almeida, R;Clausen, H
使用人UDP-半乳糖：β-N-乙酰-氨基葡萄糖β-1，3-半乳糖基转移酶的编码序列对表达序列标签(EST)进行BLAST分析，发现没有序列相同的EST，但有大量相似的EST。编制了三组与β3Gal-T1序列相似的重叠EST，并获得了这些基因的完整编码区。其中两个基因在杆状病毒系统中的表达表明，其中一个基因代表UDP-半乳糖：β-N-乙酰氨基葡萄糖β-1，3-半乳糖基转移酶(β3Gal-T2)，其动力学性质与β3Gal-T1相似。另一个基因代表UDP-半乳糖：β-N-乙酰-半乳糖胺β-1，3-半乳糖基转移酶(β3Gal-T4)，参与G(M1)/G(D1)神经节苷脂的合成，该基因与最近报道的大鼠G(D1)合成酶(Miyazaki，H.，Fukumoto，S.，Okada，M.，M.，Hasegawa，T.和Furukawa，K.(1997)J.Biol)高度相似。化学。24794-24799)，用四个同源的基因对人体器官的基因进行Northern分析，发现了不同的表达模式。β3Gal-T1在脑中表达，β3Gal-T2在脑和心脏中表达，β3Gal-T3和-T4在脑中表达更广泛。这四个基因的编码区都包含在单个外显子中。经EST定位，β3Gal-T2、-T3和-T4分别定位于1q31、3q25和6p21.3。结果表明，存在一个同源的β3-半乳糖基转移酶基因家族。
BLAST analysis of expressed sequence tags (ESTs) using the coding sequence of a human UDP-galactose:beta-N-acetyl-glucosamine beta-1,3-galactosyltransferase, designated beta 3Gal-T1, revealed no ESTs with identical sequences but a large number with similarity. Three different sets of overlapping ESTs with sequence similarities to beta 3Gal-T1 were compiled, and complete coding regions of these genes were obtained Expression of two of these genes in the Baculo virus system showed that one represented a UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase (beta 3Gal-T2) with similar kinetic properties as beta 3Gal-T1. Another gene represented a UDP-galactose:beta-N-acetyl-galactosamine beta-1,3-galactosyltransferase (beta 3Gal-T4) involved in G(M1)/G(D1) ganglioside synthesis, and this gene was highly similar to a recently reported rat G(D1) synthase (Miyazaki,H., Fukumoto, S., Okada, M., Hasegawa, T., and Furukawa, K. (1997) J. Biol. Chem. 272, 24794-24799), Northern analysis of mRNA from human organs with the four homologous cDNA revealed different expression patterns. beta 3Gal-T1 mRNA was expressed in brain, beta 3Gal-T2 was expressed in brain and heart, and beta 3Gal-T3 and -T4 were more widely expressed. The coding regions for each of the four genes were contained in single exons. beta 3Gal-T2, -T3, and -T4 were localized to 1q31, 3q25, and 6p21.3, respectively, by EST mapping. The results demonstrate the existence of a family of homologous beta 3-galactosyltransferase genes.