ASSOCIATION OF OVARIAN MALIGNANCY WITH EXPRESSION OF PLATELET-DERIVED ENDOTHELIAL-CELL GROWTH-FACTOR
ASSOCIATION OF OVARIAN MALIGNANCY WITH EXPRESSION OF PLATELET-DERIVED ENDOTHELIAL-CELL GROWTH-FACTOR
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DOI：
10.1093/jnci/86.16.1234
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发表时间：
1994-08-17
期刊：
影响因子：
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通讯作者：
BICKNELL, R
中科院分区：
文献类型：
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作者：
REYNOLDS, K;FARZANEH, F;BICKNELL, R
背景：目前，卵巢癌的发病机制仍然知之甚少，对女性卵巢癌的侵入性诊断和治疗效果不佳。尽管肿瘤学在科学和医学上取得了进步，但卵巢癌患者的总体5年生存率30%在20年内没有改变。了解卵巢癌的血管生成过程，不仅可以增加我们对卵巢癌发病机制的认识，还可能为治疗干预提供新的机会。目的：研究血小板衍生内皮细胞生长因子(胸苷磷酸化酶)、血管内皮生长因子、碱性成纤维细胞生长因子和转化生长因子-β(1)在正常卵巢和卵巢良恶性肿瘤中的表达。方法：29例择期卵巢切除术患者，4例正常卵巢，25例卵巢肿瘤(良性7例，交界性1例，恶性17例)。取材部位(高速血流区域)在手术后24小时内经阴道彩色多普勒成像进行引导。彩色血流显示组织内血流增加(即血流速度为7 cm/S)，与搏动指数小于1.0一起构成阳性扫描结果。在扫描阳性的组织中，选择血流最大的区域。在扫描阴性组织中，复杂病变选择实体区域，单纯性病变选择囊壁。随后从高流速区域(即，扫描结果为阳性的组织)或从扫描结果为阴性的组织中的实体区域或间隔中提取卵巢RNA。用核糖核酸酶保护法检测4种血管生成因子编码基因的表达。结果：2例正常卵巢(含黄体)、1例良性肿瘤和17例恶性肿瘤(加交界区)扫描阳性。扫描阳性组织与阴性组织中血小板衍化内皮细胞生长因子的表达差异有统计学意义(P
Background: At the present time, the pathogenesis of ovarian cancer remains poorly understood, with invasive diagnosis and ineffective treatment for women with the disease. Despite scientific and medical advances in oncology, the overall 5-year survival rate of 30% for ovarian cancer patients has not changed in 20 years. An understanding of the angiogenic process as it occurs in ovarian cancer would not only increase our knowledge of the pathogenesis of this cancer but also might offer novel opportunities for therapeutic intervention. Purpose: Our aim was to study the expression of messenger RNA (mRNA) coding for four putative angiogenic factors in normal ovaries and benign and malignant ovarian tumors: platelet-derived endothelial cell growth factor (thymidine phosphorylase), vascular endothelial growth factor, basic fibroblast growth factor, and transforming growth factor-beta(1). Methods: Four normal ovaries and 25 tumors (seven benign, one of borderline malignancy, and 17 malignant) were collected from 29 patients during elective oophorectomy. The site of sampling (areas of high-velocity blood flow) was directed by transvaginal color Doppler imaging performed within 24 hours of the surgery. Increased blood flow within the tissues was demonstrated by the presence of color (i.e., the velocity was >7 cm/s) and, together with a pulsatile index of less than 1.0, constituted a positive scanning result. In scan-positive tissues, the area of maximum blood flow was chosen. In scan-negative tissues, a solid area was chosen in complex lesions, or the cyst wall was chosen in simple lesions. Ovarian RNA was subsequently extracted from areas of high-velocity flow (i.e., tissues with a positive scanning result) or from solid areas or septa in tissues with a negative scanning result. A ribonuclease protection assay was used to assess the expression of mRNA coding for the four angiogenic factors. Results: Two normal ovaries (containing a corpus luteum) and one benign and 17 malignant tumors (plus the borderline) gave a positive scanning result. There was a significant difference between the expression of mRNA for platelet-derived endothelial cell growth factor between scan-positive and scan-negative tissues (P