A Hexameric Ribozyme Nanostructure Formed by Double‐Decker Assembly of a Pair of Triangular Ribozyme Trimers
A Hexameric Ribozyme Nanostructure Formed by Double‐Decker Assembly of a Pair of Triangular Ribozyme Trimers
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一对三角形核酶三聚体双层组装形成的六聚核酶纳米结构
DOI：
10.1002/cbic.202100573
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发表时间：
2022
期刊：
影响因子：
3.2
通讯作者：
Ikawa Yoshiya
中科院分区：
文献类型：
--
作者：
Yu Kai;Hidaka Kumi;Sugiyama Hiroshi;Endo Masayuki;Matsumura Shigeyoshi;Ikawa Yoshiya
天然存在的核酶的模块化结构使其成为一类有前途的结构平台，用于设计和组装三维（3D）RNA纳米结构，其中可以安装平台核酶的催化能力。我们构建并分析了具有多边形（闭合）核酶寡聚体的RNA纳米结构，其通过组装来自具有典型模块化结构的四膜虫组I内含子的单元RNA来实现。在本研究中，我们通过引入三个柱单元，使核酶三聚体二聚为三角形。用原子力显微镜观察了含有6个核酶单元的双层德克尔纳米结构的生物化学特征和结构。双层德克尔六聚体比亲本核酶三聚体表现出更高的催化活性。
The modular architecture of naturally occurring ribozymes makes them a promising class of structural platform for the design and assembly of three‐dimensional (3D) RNA nanostructures, into which the catalytic ability of the platform ribozyme can be installed. We have constructed and analyzed RNA nanostructures with polygonal‐shaped (closed) ribozyme oligomers by assembling unit RNAs derived from theTetrahymenagroup I intron with a typical modular architecture. In this study, we dimerized ribozyme trimers with a triangular shape by introducing three pillar units. The resulting double‐decker nanostructures containing six ribozyme units were characterized biochemically and their structures were observed by atomic force microscopy. The double‐decker hexamers exhibited higher catalytic activity than the parent ribozyme trimers.