An initial investigation of photocurable three-dimensional lactic acid based scaffolds in a critical-sized cranial defect
An initial investigation of photocurable three-dimensional lactic acid based scaffolds in a critical-sized cranial defect
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DOI：
10.1016/s0142-9612(02)00538-0
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发表时间：
2003-04-01
期刊：
影响因子：
14
通讯作者：
Anseth, KS
中科院分区：
文献类型：
--
作者：
Burdick, JA;Frankel, D;Anseth, KS
由多功能单体光引发聚合形成的可降解聚合物网络作为原位成型材料，特别是用于骨组织工程具有很大的潜力。在这项研究中，一个特定的化学分析方面的骨形成在一个临界尺寸的缺损模型，有和没有吸附骨诱导生长因子的存在。支架在8个月内降解，其弹性模量与松质骨相似。将一种孔隙率约为80%，孔径为45 - 150 mm的多孔支架植入大鼠颅骨缺损处。当单独植入时，支架在9周后主要由纤维组织填充，在缺损部位仅有轻度炎症。当支架释放骨诱导生长因子时，统计学上更多的骨填充支架。例如，在骨诱导释放支架中，65.8 +/- 9.4%（n = 5）的缺损填充有不透射线的组织，而在植入后9周，在未处理的缺损中，仅24.2 +/- 7.4%（n = 5）的缺损填充。这些结果不仅说明了在开发合成骨移植物时递送骨诱导因子的益处，而且说明了这些材料支持大缺损中骨浸润和发育的潜力。(C)2002爱思唯尔科技有限公司版权所有。
Degradable polymer networks formed by the photoinitiated polymerization of multifunctional monomers have great potential as in situ forming materials, especially for bone tissue engineering. In this study, one specific chemistry was analyzed with respect to bone formation in a critical-sized defect model with and without adsorbed osteoinductive growth factors present. The scaffolds degraded in similar to8 months and possessed an elastic modulus similar to that of trabecular bone. A porous scaffold fabricated with similar to80% porosity and pore diameters ranging from 45 to 150mm was implanted in a critical-sized cranial defect in rats. When implanted alone, the scaffolds were filled primarily with fibrous tissue after 9 weeks with only mild inflammation at the defect site. When the scaffolds released osteoinductive growth factors, statistically more bone filled the scaffold. For instance, 65.8 +/- 9.4% (n = 5) of the defects were filled with radiopaque tissue in the osteoinductive releasing scaffolds, whereas only 24.2 +/- 7.4% (n = 5) of the defects were filled in the untreated defects 9 weeks after implantation. These results illustrate not only the benefits of delivering osteoinductive factors when developing synthetic bone grafts, but the potential of these materials for supporting the infiltration and development of bone in large defects. (C) 2002 Elsevier Science Ltd. All rights reserved.