Differential synthesis of human placental lactogen and human chorionic gonadotropin in vitro.
Differential synthesis of human placental lactogen and human chorionic gonadotropin in vitro.
复制标题
人胎盘催乳素和人绒毛膜促性腺激素的体外差异合成。
DOI：
10.1210/endo-102-2-597
复制
发表时间：
1978
期刊：
影响因子：
4.8
通讯作者：
S. Handwergeri
中科院分区：
文献类型：
--
作者：
A. Golander;Janet Barrett;Lee Tyrey;W. Fletcher;S. Handwergeri
本研究通过在含有15%胎牛血清的最小必需培养基中培养正常足月胎盘外植体，研究了人胎盘催乳素（hPL）和人绒毛膜促性腺激素（hCG）的合成和分泌的动态。每天结束时，通过放射免疫测定法测定培养基和组织中hPL和hCG的总量，并在用25 μCi [3 H]亮氨酸脉冲1小时后的前2天内，通过[3 H]亮氨酸掺入hPL和hCG中的几个时间间隔测量新激素的合成。hPL的分泌在第1天达到高峰，第2天下降83.5 ± 9.3%，随后2天保持稳定。培养第1 ~ 2天hCG分泌量下降78.0 ± 3.7%。与hPL相反，hCG的分泌从第2天到第4天平均增加780%。在培养的第1天，由于加入了环己基琥珀酸，释放到培养基中的激素几乎全部保留。
The dynamics of the synthesis and secretion of human placental lactogen (hPL) and human chorionic gonadotropin (hCG) were investigated by culturing explants from normal term placentae in minimal essential medium with 15% fetal calf serum for periods up to 4 days. The total amounts of hPL and hCG in the media and tissues at the end of each day were determined by radioimmunoassay, and new hormone synthesis was measured by incorporation of [3H]leucine into hPL and hCG at several intervals during the first 2 days after a 1-h pulse with 25 μCi of [3H]leucine. The secretion of hPL was maximal on the 1st day, decreased by 83.5 ± 9.3% on the 2nd day, and then remained constant over the next 2 days. The secretion of hCG also decreased by 78.0 ± 3.7% from the 1st to the 2nd day of culture. In contrast to hPL, however, the secretion of hCG increased by an average of 780% from the 2nd to the 4th days. Hormone released into the medium during the 1st day in culture was almost all preformed since the addition of cyclohe...